BME103 s2013:T900 Group6 L3: Difference between revisions
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* Feature 1 - The PCR machine effectively amplifies the DNA with little error. | * Feature 1 - The PCR machine effectively amplifies the DNA with little error. | ||
* Feature 2 - The software used in the PCR testing is very easy to use. | * Feature 2 - The software used in the PCR testing is very easy to use. | ||
* Feature 3 - The | * Feature 3 - The fluorimeter gave us a good idea whether a sample was positive or negative | ||
'''DNA Measurement and Analysis Protocol''' | |||
<!-- Create a step-by-step procedure for measuring DNA amplification in the PCR reactions. Your instructions should include everything from diluting the samples in SYBR Green, to placing the drops onto the fluorimeter (if your group is using the fluorimeter), to collecting and processing images in Image J. Don't forget to provide instructions on how to set up the calf thymus DNA samples for calibration, and how to convert INTDEN values into concentrations.---> | <!-- Create a step-by-step procedure for measuring DNA amplification in the PCR reactions. Your instructions should include everything from diluting the samples in SYBR Green, to placing the drops onto the fluorimeter (if your group is using the fluorimeter), to collecting and processing images in Image J. Don't forget to provide instructions on how to set up the calf thymus DNA samples for calibration, and how to convert INTDEN values into concentrations.---> | ||
# Step 1: Gather materials and safety equipment | # Step 1: Gather materials and safety equipment | ||
# Step 2: Put | # Step 2: Put fluorimeter in dark box, keep one hatch open for ease of access | ||
# Step 3: Level the phone camera lens with | # Step 3: Level the phone camera lens with fluorimeter | ||
# Step 4: Calibrate | # Step 4: Calibrate fluorimeter with water | ||
# Step 5: Dilute samples with SYBR green | # Step 5: Dilute samples with SYBR green | ||
# Step 6: Run | # Step 6: Run fluorimeter again | ||
# Step 7: Take picture and repeat with the different samples | # Step 7: Take picture and repeat with the different samples | ||
# Step 8: Open image J | # Step 8: Open image J |
Revision as of 19:56, 15 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 3 WRITE-UPOriginal System: PCR ResultsPCR Test Results
* Ave. INTDEN = Average of ImageJ integrated density values from three Fluorimeter images
Bayes Theorem equation: P(A|B) = P(B|A) * P(A) / P(B)
Calculation 3: The probability that the patient will develop cancer, given a cancer DNA sequence.
New System: Design StrategyWe concluded that a good system Must Have:
New System: Machine/ Device EngineeringSYSTEM DESIGN
KEY FEATURES We chose to include these new features
New System: ProtocolsDESIGN We chose to include these new approaches/ features
MATERIALS Supplied in Kit
PROTOCOLS
We chose keep the protocols the same as the original system
DNA Measurement and Analysis Protocol
New System: Research and DevelopmentBACKGROUND
DESIGN
New System: Software[THIS SECTION IS OPTIONAL. If your team has creative ideas for new software, and new software is a key component included in your new protocols, R&D, or machine design, you may describe it here. You will not receive bonus points, but a solid effort may raise your overall page layout points. If you decide not to propose new software, please delete this entire section, including the ==New System: Software== header.]
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