BME103 s2013:T900 Group7

BME 103 Fall 2012	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Amanda Seaney Role(s) Name: Carissa Henriksen Role(s)Protocol Name: Nathan Scheuer Role: Open PCR machine Engineer Name: student Role(s) Name: student Role(s) Name: student Role(s) LAB 1 WRITE-UP Initial Machine Testing The Original Design (Add image of the full OpenPCR machine here, from the Week 9 exercise. Write a paragraph description for visitors who have no idea what this is) Experimenting With the Connections When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer) Test Run (Write the date you first tested Open PCR and your experience(s) with the machine) Protocols Thermal Cycler Program DNA Sample Set-up Tube: A, Positive Control: Cancer DNA template Tube: B, Patient 1 ID: 43825, Replicate: 1 Tube: C, Patient 1 ID: 43825, Replicate: 2 Tube: D, Patient 1 ID: 43825, Replicate: 3 Tube: E, Negative Control: No DNA template Tube: F, Patient 2 ID: 12079, Replicate: 1 Tube: G, Patient 2 ID: 12079, Replicate: 2 Tube: H, Patient 2 ID: 12079, Replicate: 3 DNA Sample Set-up Procedure Obtain 50 microliters of sample DNA into a micropipet. Place DNA sample into provided enzyme mix. Place tubes into PCR machine, which connected to the computer. PCR Reaction Mix What is in the PCR reaction mix? Answer: Taq DNA polymerase, magnesium chloride, dNTP's, forward and reverse primers DNA/ primer mix What is in the DNA/ primer mix? Answer: Patient's DNA Research and Development Specific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)