BME103 s2013:T900 Group8: Difference between revisions
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==Research and Development== | ==Research and Development== | ||
'''Specific Cancer Marker Detection - The Underlying Technology'''<br> | |||
The goal of this experiment is to understand the DNA amplification process in order to detect cancerous genes when given Template DNA; otherwise known as DNA taken from the patient. DNA amplification will allow the cancerous genes to replicate. The cancerous gene will produce a positive result, while the non-cancer gene will give a negative result, because the primers are designed to amplify cancerous DNA. Therefore, the cancerous mutation cannot bind to normal DNA, ultimately meaning that amplification cannot occur. | |||
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''These “3 steps” are repeated for 30 cycles, each step taking about 30 seconds.''<br> | ''These “3 steps” are repeated for 30 cycles, each step taking about 30 seconds.''<br> | ||
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• What happens? <br> | • What happens? <br> | ||
o The bases need to me detected, in order to do so “melting” or unzipping needs to DNA will expose those base pairs. | o The bases need to me detected, in order to do so “melting” or unzipping needs to DNA will expose those base pairs. | ||
o When the base pairs are exposed the primers will create a forward and reverse strand appropriately | o When the base pairs are exposed the primers will create a forward and reverse strand appropriately. The primers are short pieces (about 20 bases pairs long) of DNA that are synthesized DNA that binds to the Template DNA while directing the new strand to be made. | ||
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'''Step 2:'''The mixture is cooled down to 57 degrees Celsius<br> | '''Step 2:'''The mixture is cooled down to 57 degrees Celsius<br> | ||
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• What happens?<br> | • What happens?<br> | ||
o The Taq Polymerase detect where the template DNA and primer and uses the template DNA strand and primer to build a new copy of DNA | o The Taq Polymerase and Magnesium Chloride will detect where the template DNA and primer and uses the template DNA strand and primer to build a new copy of DNA<br> | ||
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Revision as of 17:24, 23 March 2013
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program DNA Sample Set-up
DNA Sample Set-up Procedure
PCR Reaction Mix
DNA/ primer mix
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology The goal of this experiment is to understand the DNA amplification process in order to detect cancerous genes when given Template DNA; otherwise known as DNA taken from the patient. DNA amplification will allow the cancerous genes to replicate. The cancerous gene will produce a positive result, while the non-cancer gene will give a negative result, because the primers are designed to amplify cancerous DNA. Therefore, the cancerous mutation cannot bind to normal DNA, ultimately meaning that amplification cannot occur.
These “3 steps” are repeated for 30 cycles, each step taking about 30 seconds. • What happens? • What happens? • What happens?
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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