BME103 s2013:T900 Group8: Difference between revisions
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'''Specific Cancer Marker Detection - The Underlying Technology'''<br> | '''Specific Cancer Marker Detection - The Underlying Technology'''<br> | ||
The goal of this experiment is to understand the DNA amplification process in order to detect cancerous genes when given Template DNA; otherwise known as DNA taken from the patient. DNA amplification will allow the cancerous genes to replicate. The cancerous gene will produce a positive result, while the non-cancer gene will give a negative result, because the primers are designed to amplify cancerous DNA. Therefore, the cancerous mutation cannot bind to normal DNA, ultimately meaning that amplification cannot occur. | The goal of this experiment is to understand the DNA amplification process in order to detect cancerous genes when given Template DNA; otherwise known as DNA taken from the patient. DNA amplification will allow the cancerous genes to replicate. The cancerous gene will produce a positive result, while the non-cancer gene will give a negative result, because the primers are designed to amplify cancerous DNA. Therefore, the cancerous mutation cannot bind to normal DNA, ultimately meaning that amplification cannot occur.<br> | ||
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'''Functions of each component of the PCR Chain Reaction:'''<br> | |||
''Template DNA:'' The DNA taken from the patient<br> | |||
''Primers:'' Short (20 base pairs) pieces of synthesized DNA<br> | |||
''Taq Polymerase:'' is an enzyme that detects the primer-template DNA and snaps the dNtp's back to DNA<br> | |||
''Magnesium Chloride (MgCl2):'' serves as a "co-factor" for Taq Polymerase<br> | |||
''dNTP's:'' Once the DNA is "unzipped"and recombined the dNTP's are the unincorporated bases<br> | |||
''The following “3 steps” are repeated for 30 cycles, each step taking about 30 seconds.''<br> | ''The following “3 steps” are repeated for 30 cycles, each step taking about 30 seconds.''<br> |
Revision as of 21:56, 25 March 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design (Write the date you first tested Open PCR and your experience(s) with the machine)
Protocols
Our job as for Experiment Protocol Planner was to :
We have educated our self via “PCR Virtual Lab” that can be found at http://openpcr.ord/use-it/ then downloaded and test software that was responsible for taking the results from PCR machine and displaying those results on PC, as well as to make commends to the PCR machine. This software also gave us real time update of the PCR machine PCR experiment summery
"Contains" describes what sample was inserted in the test tube DNA Sample Set-up Procedure 1. Step 1 : Acquire Materials
2. Step 2 : Mix dyes
3. Step 3 : Prepare the samples, and label the samples (tables is shown above)
6. Step 6 : Start the PCR machine using software PCR Reaction Mix
DNA/ primer mix
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology The goal of this experiment is to understand the DNA amplification process in order to detect cancerous genes when given Template DNA; otherwise known as DNA taken from the patient. DNA amplification will allow the cancerous genes to replicate. The cancerous gene will produce a positive result, while the non-cancer gene will give a negative result, because the primers are designed to amplify cancerous DNA. Therefore, the cancerous mutation cannot bind to normal DNA, ultimately meaning that amplification cannot occur. The following “3 steps” are repeated for 30 cycles, each step taking about 30 seconds. • What happens? • What happens? • What happens?
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