BME103 s2013:T900 Group8: Difference between revisions
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==Research and Development== | |||
'''Specific Cancer Marker Detection - The Underlying Technology'''<br> | '''Specific Cancer Marker Detection - The Underlying Technology'''<br> |
Revision as of 16:57, 23 March 2013
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsThermal Cycler Program DNA Sample Set-up
DNA Sample Set-up Procedure
PCR Reaction Mix
DNA/ primer mix
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology These “3 steps” are repeated for 30 cycles, each step taking about 30 seconds. • What happens? • What happens? • What happens?
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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