BME103 s2013:T900 Group8: Difference between revisions
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# Analyze the results | # Analyze the results | ||
We have educated our self via “PCR Virtual Lab” that can be found at http://openpcr.ord/use-it/ then downloaded and test software that was responsible for taking the results from PCR machine and displaying those results on PC, as well as to make commends to the PCR machine. This software also gave us real time update of the PCR machine | We have educated our self via “PCR Virtual Lab” that can be found at http://openpcr.ord/use-it/ then downloaded and test software that was responsible for taking the results from PCR machine and displaying those results on PC, as well as to make commends to the PCR machine. This software also gave us real time update of the PCR machine <br> | ||
Second and most important part of to prepare our sample. Our sample consisted of two rows of four samples. The table representing the samples is shown below. In this part we have also created a total of 8 different dyes also shown below. | Second and most important part of to prepare our sample. Our sample consisted of two rows of four samples. The table representing the samples is shown below. In this part we have also created a total of 8 different dyes also shown below. <br> | ||
The third part was to take the results from the PCR machine and analyze them with the rest of our team, via software. called | The third part was to take the results from the PCR machine and analyze them with the rest of our team, via software. called | ||
'''Thermal Cycler Program'''<br> | '''Thermal Cycler Program'''<br> | ||
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'''DNA Sample Set-up Procedure''' | '''DNA Sample Set-up Procedure''' | ||
1. Step 1 : Acquire Materials | |||
{| {{table}} | |||
|- | |||
| Materials : | |||
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| - A set of four dyes (blue, green, yellow, red) | |||
|- | |||
| - Sample containing (+,-,P1,P1,P1,P2,P2,P2) | |||
|- | |||
| - Pipette and tips | |||
|- | |||
| - two rows of empty test tubes | |||
|} | |||
2. Step 2 : Mix dyes | |||
{| {{table}} | |||
|- | |||
| MIX: | |||
|- | |||
| blue 50μL || blue 50μL | |||
|- | |||
| blue 50μL || yellow 50μL | |||
|- | |||
| blue 50μL || green 50μL | |||
|- | |||
| yellow 50μL || yellow 50μL | |||
|- | |||
| yellow 50μL || green 50μL | |||
|- | |||
| red 50μL || red 50μL | |||
|- | |||
| red 50μL || green 50μL | |||
|- | |||
| green 50μL || green 50μL | |||
|} | |||
3. Step 3 : Prepare the samples, and label the samples (tables is shown above) <br> | |||
4. Step 4 : allow other team members to start PCR machine and then load the samples into the machine | |||
'''PCR Reaction Mix''' | '''PCR Reaction Mix''' |
Revision as of 11:28, 25 March 2013
BME 103 Fall 2012 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged (part 3) from (part 6), the machine ... (did what? fill in your answer) When we unplugged the white wire that connects (part 6) to (part 2), the machine ... (did what? fill in your answer)
(Write the date you first tested Open PCR and your experience(s) with the machine)
ProtocolsExperimental protocol planner summery Our job as for Experiment Protocol Planner was to :
We have educated our self via “PCR Virtual Lab” that can be found at http://openpcr.ord/use-it/ then downloaded and test software that was responsible for taking the results from PCR machine and displaying those results on PC, as well as to make commends to the PCR machine. This software also gave us real time update of the PCR machine DNA Sample Set-up
"Contains" describes what sample was inserted in the test tube DNA Sample Set-up Procedure 1. Step 1 : Acquire Materials
2. Step 2 : Mix dyes
3. Step 3 : Prepare the samples, and label the samples (tables is shown above) PCR Reaction Mix
DNA/ primer mix
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology The goal of this experiment is to understand the DNA amplification process in order to detect cancerous genes when given Template DNA; otherwise known as DNA taken from the patient. DNA amplification will allow the cancerous genes to replicate. The cancerous gene will produce a positive result, while the non-cancer gene will give a negative result, because the primers are designed to amplify cancerous DNA. Therefore, the cancerous mutation cannot bind to normal DNA, ultimately meaning that amplification cannot occur.
The following “3 steps” are repeated for 30 cycles, each step taking about 30 seconds. • What happens? • What happens? • What happens?
(BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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