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The PCR Machine otherwise known as a Thermocycler or a DNA Amplifyer is use by scientists to create a vast quanity of a specific sequence of DNA. This method is also used widely for biological and medical applicaions. This process relies on the thermal cycles to amplify the DNA to then be able to replicated it. The system is run by means of a software downloaded to either a desktop or laptop. By connecting the PCR machine to a computer it is then possible to run a test with the unique specifications needed; such as tempurature and number of cycles.<br><br>'''Experimenting With The Connections'''[[Image:PCR_group3_.png|thumb|frame|left|A description of the image goes here]] When taking apart the PCR Machine <br><br>'''Test Run''' | The PCR Machine otherwise known as a Thermocycler or a DNA Amplifyer is use by scientists to create a vast quanity of a specific sequence of DNA. This method is also used widely for biological and medical applicaions. This process relies on the thermal cycles to amplify the DNA to then be able to replicated it. The system is run by means of a software downloaded to either a desktop or laptop. By connecting the PCR machine to a computer it is then possible to run a test with the unique specifications needed; such as tempurature and number of cycles.<br><br>'''Experimenting With The Connections'''[[Image:PCR_group3_.png|thumb|frame|left|A description of the image goes here]] When taking apart the PCR Machine there are pieces that if not connected properly or were not working apropriatly would cause the test to be inacurate and result in unreliable data.<br> By umpluging the LCD screen from everything else truned off the screen and we were unable to see the process that the machine is running.<br><br>'''Test Run''' | ||
The first test date was March 5. It began around 10:00am and completed the 4 minute test cycle with no issues.<br><br> | The first test date was March 5. It began around 10:00am and completed the 4 minute test cycle with no issues.<br><br> |
Revision as of 23:03, 25 March 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 1 WRITE-UPInitial Machine Testing
Experimenting With The Connections When taking apart the PCR Machine there are pieces that if not connected properly or were not working apropriatly would cause the test to be inacurate and result in unreliable data. By umpluging the LCD screen from everything else truned off the screen and we were unable to see the process that the machine is running. Test Run The first test date was March 5. It began around 10:00am and completed the 4 minute test cycle with no issues.
Protocols
Our job as for Experiment Protocol Planner was to :
We have educated our self via “PCR Virtual Lab” that can be found at http://openpcr.ord/use-it/ then downloaded and test software that was responsible for taking the results from PCR machine and displaying those results on PC, as well as to make commends to the PCR machine. This software also gave us real time update of the PCR machine PCR experiment summery
"Contains" describes what sample was inserted in the test tube DNA Sample Set-up Procedure 1. Step 1 : Acquire Materials
2. Step 2 : Mix dyes
3. Step 3 : Prepare the samples, and label the samples (tables is shown above)
6. Step 6 : Start the PCR machine using software PCR Reaction Mix
DNA/ primer mix
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology The goal of this experiment is to understand the DNA amplification process in order to detect cancerous genes when given Template DNA; otherwise known as DNA taken from the patient. DNA amplification will allow the cancerous genes to replicate. The cancerous gene will produce a positive result, while the non-cancer gene will give a negative result, because the primers are designed to amplify cancerous DNA. Therefore, the cancerous mutation cannot bind to normal DNA, ultimately meaning that amplification cannot occur. The following “3 steps” are repeated for 30 cycles, each step taking about 30 seconds. • What happens? • What happens? • What happens?
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