OUR TEAM
Name: student
Name: student
Name: student
Name: student
Name: student
Name: student
LAB 2 WRITE-UP
Background Information
SYBR Green Dye
[A short summary describing SYBR green dye]
Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Tech Specs
Phone :
iphone 4
Tech specs
" 5-megapixel iSight camera
Video recording, HD (720p) up to 30 frames per second with audio
VGA-quality photos and video at up to 30 frames per second with the front camera
Tap to focus video or still images
LED flash
Photo and video geotagging "
Source of Tech specs
http://www.apple.com/iphone/iphone-4/specs.html
Smart Phone Camera Settings
Settings :
Settings used in lab
Observations
Flash:
Turned off
N/A
ISO setting:
ISO setting 80-1000
No control over ISO settings
White Balance:
Auto White balance
No control over white balance
Exposure:
Auto exposure
No control over exposure
Saturation:
Very high
No control over saturation
Contrast:
Very high
No control over contrast
Source:
http://www.imaging-resource.com/PRODS/IPHONE4/IPHONE4A.HTM
This source was used to find out what were the defult settings of iphone 4
[No additional phones or cameras used]
Calibration
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
Distance between the smart phone cradle and drop =
[See worksheet page 5]
Solutions Used for Calibration [See worksheet page 5]
row 1 cell 1
row 1 cell 2
row 1 cell 3
row 1 cell 4
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
row 3 cell 1
row 3 cell 2
row 3 cell 3
row 3 cell 4
[Add more rows as needed]
Placing Samples onto the Fluorimeter
[Step one, in your OWN words]
[Step two, in your own words]
[Step three, in your own words]
[etc.]
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Image J Values for All Samples [See worksheet page 5]
row 1 cell 1
row 1 cell 2
row 1 cell 3
row 1 cell 4
row 1 cell 5
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
row 2 cell 5
row 3 cell 1
row 3 cell 2
row 3 cell 3
row 3 cell 4
row 2 cell 5
[Add more rows as needed]
Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]