BME103 s2013:T900 Group8 L2: Difference between revisions
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The objectives of the lab were met. The flourimeter technique was mastered and calibrated. The formula to determine the magnitude of the light from a given concentration was found to be y = 4.0 x 10^6 (x) - 272558[2.7 x 10^6]. This can be used to find the concentration of DNA in an unknown sample by measuring the magnitude of light coming off the drop. | The objectives of the lab were met. The flourimeter technique was mastered and calibrated. The formula to determine the magnitude of the light from a given concentration was found to be y = 4.0 x 10^6 (x) - 272558[2.7 x 10^6]. This can be used to find the concentration of DNA in an unknown sample by measuring the magnitude of light coming off the drop using the freehand tool mentioned above. | ||
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Revision as of 08:37, 2 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground InformationSYBR Green Dye SYBR Green I is a dye that is used as a nucleic acid stain in several fields such as biochemistry, and molecualr biology. SYBR Green I can be diluted and a small amount of it can go a long way. This dye was useful in this experiment because, when the drop of DNA is put on the glass slide there is a blue ray of light that is turned on, which then passes through the drop. The way that this compund, SYBR Green, works is by absorbing the blue light and emitting green light. When we taking a picture of the drop under dark conditions the green emmited light can be visible. This compound is part of the cyanine synthetic dye family, this words comes from the word "cyan" which is blue-green. That this why green light is emitted. Single-Drop Fluorimeter
Procedure
Smart Phone Camera Settings
Calibration Distance and angles between the smart phone and =
Placing Samples onto the Fluorimeter Procedures
Data AnalysisRepresentative Images of Samples A drawn circle using the freehand tool for a sample with no DNA'' A drawn circle using the freehand tool for a sample with DNA (positive signal)
Image J Values for All Samples [See worksheet page 5]
In order for a linear pattern effect the last set of data is not included. If included, a curve would have been created due to human error during experimentation.
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