BME103 s2013:T900 Group8 L2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: student Name: student Name: student Name: student Name: student Name: student LAB 2 WRITE-UP Background Information SYBR Green Dye [A short summary describing SYBR green dye] Single-Drop Fluorimeter [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points] How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet] Procedure Smart Phone Camera Tech Specs Phone : iphone 4 Tech specs " 5-megapixel iSight camera Video recording, HD (720p) up to 30 frames per second with audio VGA-quality photos and video at up to 30 frames per second with the front camera Tap to focus video or still images LED flash Photo and video geotagging " Source of Tech specs http://www.apple.com/iphone/iphone-4/specs.html Smart Phone Camera Settings Settings : Settings used in lab Observations Flash: Turned off N/A ISO setting: ISO setting 80-1000 No control over ISO settings White Balance: Auto White balance No control over white balance Exposure: Auto exposure No control over exposure Saturation: Very high No control over saturation Contrast: Very high No control over contrast Source: http://www.imaging-resource.com/PRODS/IPHONE4/IPHONE4A.HTM This source was used to find out what were the defult settings of iphone 4 [No additional phones or cameras used] Calibration Open PCR Machine used in Lab Distance and angles between the smart phone and = candle = 3.62cm table = 5.67cm angle from camera to drop = approx 90° Solutions Used for Calibration Solution Concetration of SYBR green 1 Water 2 0.25 μg/mL 3 0.05 μg/mL 4 1.0 μg/mL 5 2.0 μg/mL 6 5.0 μg/mL Placing Samples onto the Fluorimeter [Step one, in your OWN words] [Step two, in your own words] [Step three, in your own words] [etc.] Data Analysis Representative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA] [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] Image J Values for All Samples [See worksheet page 5] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5 [Add more rows as needed] Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]