BME103 s2013:T900 Group8 L2

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| [[Image:BME103student.jpg|100px|thumb|Name: Josh Snyder<br>Role: Machine Tester]]
| [[Image:BME103student.jpg|100px|thumb|Name: Josh Snyder<br>Role: Machine Tester]]
| [[Image:BME103student.jpg|100px|thumb|Name: Adam Pak<br>Role:Experimental Protocol Planner]]
| [[Image:BME103student.jpg|100px|thumb|Name: Adam Pak<br>Role:Experimental Protocol Planner]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Sunhine Silvas,br>Role: Machine Tester]]
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| [[Image:BME103student.jpg|100px|thumb|Name: Sunhine Silvas<br>Role: Machine Tester]]
| [[Image:BME103student.jpg|100px|thumb|Name: Renee Tran<br>Role:Research and Development Scientist]]
| [[Image:BME103student.jpg|100px|thumb|Name: Renee Tran<br>Role:Research and Development Scientist]]
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Revision as of 23:23, 1 April 2013

BME 103 Spring 2013 Home
People
Lab Write-Up 1
Lab Write-Up 2
Lab Write-Up 3
Course Logistics For Instructors
Photos
Wiki Editing Help
Image:BME494_Asu_logo.png


Contents

OUR TEAM

Name: Anthony ZingaleRole: Experimental Protocol Planner
Name: Anthony Zingale
Role: Experimental Protocol Planner
Name: Josh SnyderRole: Machine Tester
Name: Josh Snyder
Role: Machine Tester
Name: Adam PakRole:Experimental Protocol Planner
Name: Adam Pak
Role:Experimental Protocol Planner
Name: Sunhine SilvasRole: Machine Tester
Name: Sunhine Silvas
Role: Machine Tester
Name: Renee TranRole:Research and Development Scientist
Name: Renee Tran
Role:Research and Development Scientist


LAB 2 WRITE-UP

Background Information

SYBR Green Dye
[A short summary describing SYBR green dye]


Single-Drop Fluorimeter
[A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]


How the Fluorescence Technique Works
[In your own words, a summary of the information from page 9 of the worksheet]



Procedure


Smart Phone Camera Tech Specs

Phone : iphone 4
Tech specs "
5-megapixel iSight camera

Video recording, HD (720p) up to 30 frames per second with audio

VGA-quality photos and video at up to 30 frames per second with the front camera

Tap to focus video or still images

LED flash

Photo and video geotagging
"

Source of Tech specs http://www.apple.com/iphone/iphone-4/specs.html

Smart Phone Camera Settings

Settings : Settings used in lab Observations
Flash: Turned off N/A
ISO setting: ISO setting 80-1000 No control over ISO settings
White Balance: Auto White balance No control over white balance
Exposure: Auto exposure No control over exposure
Saturation: Very high No control over saturation
Contrast: Very high No control over contrast
Source: http://www.imaging-resource.com/PRODS/IPHONE4/IPHONE4A.HTM This source was used to find out what were the defult settings of iphone 4


  • No additional phones or cameras used


Calibration

Diagram of the set up
Diagram of the set up

Distance and angles between the smart phone and =

  • candle = 3.62cm
  • table = 5.67cm
  • angle from camera to drop = approx 90°


Solutions Used for Calibration

Solution Concetration of SYBR green
1 None (Water)
2 0.25 micrograms per ml
3 0.05 micrograms per ml
4 1.0 micrograms per ml
5 2.0 micrograms per ml
6 5.0 micrograms per ml


Placing Samples onto the Fluorimeter Procedures

  • Aquire following materials :
Materials :
Set of read SYBR green(5 samples)
Water
pipette
Superhydrophobic surface
cradle
Smart phone
Box
plastic base
small box to set up the phone
  1. Calibrate phone
  2. Carefully palce one drop of sample onto Superhydrophobic surface
  3. Place Superhydrophobic surface onto cradle
  4. Place the big box over cradle to create a dark room
  5. Set up smart phone using plastic base
  6. Take a picture
  7. Repeat steps 2-6 for all 6 samples
  8. Measure distances of phone to table and to cradle
  9. Analyze pictures and write down the observations



Data Analysis

Representative Images of Samples

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]

[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]


Image J Values for All Samples [See worksheet page 5]

row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5
row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5
row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5

[Add more rows as needed]


Fitting a Straight Line
[Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]




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