BME103 s2013:T900 Group8 L2

BME 103 Spring 2013	Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help
OUR TEAM Name: Anthony Zingale Role: Experimental Protocol Planner Name: Josh Snyder Role: Machine Tester Name: Adam Pak Role:Experimental Protocol Planner Name: Sunhine Silvas Role: Machine Tester Name: Renee Tran Role:Research and Development Scientist LAB 2 WRITE-UP Background Information SYBR Green Dye molecluar diagram of SYBR Green I SYBR Green I is a dye that is used as a nucleic acid stain in several fields such as biochemistry, and molecualr biology. SYBR Green I can be diluted and a small amount of it can go a long way. This dye was useful in this experiment because, when the drop of DNA is put on the glass slide there is a blue ray of light that is turned on, which then passes through the drop. The way that this compund, SYBR Green, works is by absorbing the blue light and emitting green light. When we taking a picture of the drop under dark conditions the green emmited light can be visible. This compound is part of the cyanine synthetic dye family, this words comes from the word "cyan" which is blue-green. That this why green light is emitted. Single-Drop Fluorimeter [Add a PHOTO for bonus points] The Single-Drop Fluorimeter is a box shaped device that shines a specific light spectrum to activate the florescent dye in the sample.The light is turned on by a switch on the side of the devices. The sample is placed on specially made glass slide that is perforated and hydro-phobic, the slide can then be placed into the Fluorimeter. How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet] This method is used to examine and analyze the protiens, nucleic acids, ligands, and other interactions in order to study the protine orientations and actions. Procedure Smart Phone Camera Tech Specs Phone : iphone 4 Tech specs " 5-megapixel iSight camera Video recording, HD (720p) up to 30 frames per second with audio VGA-quality photos and video at up to 30 frames per second with the front camera Tap to focus video or still images LED flash Photo and video geotagging " Source of Tech specs http://www.apple.com/iphone/iphone-4/specs.html Smart Phone Camera Settings Settings : Settings used in lab Observations Flash: Turned off N/A ISO setting: ISO setting 80-1000 No control over ISO settings White Balance: Auto White balance No control over white balance Exposure: Auto exposure No control over exposure Saturation: Very high No control over saturation Contrast: Very high No control over contrast Source: http://www.imaging-resource.com/PRODS/IPHONE4/IPHONE4A.HTM This source was used to find out what were the defult settings of iphone 4 No additional phones or cameras used Calibration Diagram of the set up Distance and angles between the smart phone and = candle = 3.62cm table = 5.67cm angle from camera to drop = approx 90° Solutions Used for Calibration Solution Concetration of SYBR green 1 None (Water) 2 0.25 micrograms per ml 3 0.05 micrograms per ml 4 1.0 micrograms per ml 5 2.0 micrograms per ml 6 5.0 micrograms per ml Placing Samples onto the Fluorimeter Procedures Aquire following materials : Materials : Set of read SYBR green(5 samples) Water pipette Superhydrophobic surface cradle Smart phone Box plastic base small box to set up the phone Calibrate phone Carefully palce one drop of sample onto Superhydrophobic surface Place Superhydrophobic surface onto cradle Place the big box over cradle to create a dark room Set up smart phone using plastic base Take a picture Repeat steps 2-6 for all 6 samples Measure distances of phone to table and to cradle Analyze pictures and write down the observations Data Analysis Representative Images of Samples [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]'' [Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)] Image J Values for All Samples [See worksheet page 5] row 1 cell 1 row 1 cell 2 row 1 cell 3 row 1 cell 4 row 1 cell 5 row 2 cell 1 row 2 cell 2 row 2 cell 3 row 2 cell 4 row 2 cell 5 row 3 cell 1 row 3 cell 2 row 3 cell 3 row 3 cell 4 row 2 cell 5 [Add more rows as needed] Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]