BME103 s2013:T900 Group8 L3: Difference between revisions
Line 191: | Line 191: | ||
<br> | <br> | ||
4.Pre-heat PCR machine to 95 degree then place the prepared samples using standard parameters. | 4.Pre-heat PCR machine to 95 degree then place the prepared samples using standard parameters. | ||
<br> | |||
<br> | <br> | ||
'''General Guidelines for Amplification by PCR''' | '''General Guidelines for Amplification by PCR''' | ||
<br> | |||
<br> | <br> | ||
1. Acquire PCR machine and inspect its elements to ensure that the machine is read to be used<br> | 1. Acquire PCR machine and inspect its elements to ensure that the machine is read to be used<br> | ||
this will also require that a test runs are done to ensure that the PCR machine is ready to <br> | this will also require that a test runs are done to ensure that the PCR machine is ready to <br> | ||
go through appropriated cycles | go through appropriated cycles | ||
<br> | |||
<br> | <br> | ||
2.Connect PCR machine to PC via USB and using Thermal Cycler Program specify the cycles that<br> | 2.Connect PCR machine to PC via USB and using Thermal Cycler Program specify the cycles that<br> | ||
are being runned. | are being runned. cycles that must be plugged into the software are following | ||
{| {{table}} | |||
|- style="background:#f0f0f0;" | |||
| Stage || Cycles || Heat (Celsius) || time | |||
|- | |||
| 1 || 1 || 95 || 3 minutes | |||
|- | |||
| 2 || 35 || 95 to 52 to 72 || each 30 seconds | |||
|- | |||
| 3 || 1 || 72 || 3 minutes | |||
|} | |||
3.place two rows of four tubes into PCR that were prepared before, using PC click start and <br> | |||
allow time for the tests to take place leaving the PCR in a controlled environmental | |||
|| | |||
'''Using SYBR Green Dye''' | |||
<br> | |||
<br> | <br> | ||
Reagents supplied by the user : | |||
|| 2 | *SYBR Green I | ||
*DNA being tested | |||
<br> | |||
1.prepare the set up by setting up Single-Drop Fluorimeter then set a perforated and hydro-phobic <br> | |||
glass on the fluorimeter. the entire set uo should be covered by a box to ensure that the picture <br> | |||
exclude outside light. then platform for the camera should be made so that the camera can is perpendicular<br> | |||
and looks straight on the sample drop on the flourimeter that will be tested. | |||
<br> | |||
<br> | |||
2.prepare the sample by mixing following samples, the solutions are acquired from the PCR machine that <br> | |||
was run previously. | |||
{| {{table}} width=700 | |||
|-style="background:#f0f0f0;" | |||
| Solution || Concetration of SYBR green | |||
|- | |||
| 1 || None (Water) | |||
|- | |||
| 2 || 0.25 micrograms per ml | |||
|- | |||
| 3 || 0.05 micrograms per ml | |||
|- | |||
| 4 || 1.0 micrograms per ml | |||
|- | |||
| 5 || 2.0 micrograms per ml | |||
|- | |- | ||
| | | 6 || 5.0 micrograms per ml | ||
|} | |||
|} | |} | ||
Revision as of 22:18, 15 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAMLAB 3 WRITE-UPOriginal System: PCR ResultsPCR Test Results
* Ave. INTDEN = Average of ImageJ integrated density values from three Fluorimeter images
Bayes Theorem equation: P(A|B) = P(B|A) * P(A) / P(B)
Calculation 3: The probability that the patient will develop cancer, given a cancer DNA sequence.
New System: Design StrategyWe concluded that a good system Must Have:
New System: Machine/ Device EngineeringSYSTEM DESIGN
KEY FEATURES We chose to include these new features
[OR] We chose keep the devices the same as the original system
INSTRUCTIONS
New System: ProtocolsDESIGN We chose to include these new approaches/ features
[OR] We chose keep the protocols the same as the original system
New System: Research and DevelopmentBACKGROUND
DESIGN
New System: Software[THIS SECTION IS OPTIONAL. If your team has creative ideas for new software, and new software is a key component included in your new protocols, R&D, or machine design, you may describe it here. You will not receive bonus points, but a solid effort may raise your overall page layout points. If you decide not to propose new software, please delete this entire section, including the ==New System: Software== header.]
|