* Ave. INTDEN = Average of ImageJ integrated density values from three Fluorimeter images
Bayesian Statistics
These following conditional statistics are based upon all of the DNA detection system results that were obtained in the PCR lab for 20 hypothetical patients who were diagnosed as either having cancer or not having cancer.
Calculation 1: The probability that the sample actually has the cancer DNA sequence, given a positive diagnostic signal.
A = [text description] = [frequency shown as a fraction] = [final numerical value]
B = [text description] = [frequency shown as a fraction] = [final numerical value]
P (B|A) = [text description] = [frequency shown as a fraction] = [final numerical value]
P(A|B) = [answer]
Calculation 3: The probability that the patient will develop cancer, given a cancer DNA sequence.
A = [text description] = [frequency shown as a fraction] = [final numerical value]
B = [text description] = [frequency shown as a fraction] = [final numerical value]
P (B|A) = [text description] = [frequency shown as a fraction] = [final numerical value]
P(A|B) = [answer]
New System: Design Strategy
We concluded that a good system Must Have:
[Must have #1 - A "must have" for our new devise is having the results be easy to determine. The reason for this would be that it would allow for more users of the devise and give them a simple to use product. We did not want to complicate the system and thus leading to users not wanting to use the product, our goal is to simplify the PCR machine to be more user friendly.]
[Must have #2 - why? short, ~4 or 5 sentences]
We concluded that we would Want a good system to have:
[Want #1 - Something that we wanted to include in our new design is to keep it portable and compact. When thinking about who would use the machine and where, there were many different situations that came up. Although the machine will be used in a lab or an office it will be able to take it other place without having to worry about weather the devise can with stand the situations it is in. This feature also is helpful because when storing the machine it is not very difficult but simply and easy.]
[Want #2 - why? short, ~4 or 5 sentences]
We concluded that a good system Must Not Have:
[Must Not Have #1 - why? short, ~4 or 5 sentences]
[Must Not Have #2 - why? short, ~4 or 5 sentences]
We concluded that a good system Should Avoid:
[Should Avoid #1 - why? short, ~4 or 5 sentences]
[Should Avoid #2 - why? short, ~4 or 5 sentences]
New System: Machine/ Device Engineering
SYSTEM DESIGN
KEY FEATURES
We chose to include these new features
Feature 1 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
Feature 2 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
Etc.
[OR]
We chose keep the devices the same as the original system
Feature 1 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
Feature 2 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
Etc.
INSTRUCTIONS
New System: Protocols
DESIGN
We chose to include these new approaches/ features
Feature 1 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
Feature 2 - explanation of how this addresses any of the specifications in the "New System: Design Strategy" section
Etc.
[OR]
We chose keep the protocols the same as the original system
Feature 1 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
Feature 2 - explanation of how a pre-existing feature addresses any of the specifications in the "New System: Design Strategy" section
Etc.
Standard Application
Reagents supplied by the user :
Template DNA
Primer
DNA polymerase
MgCl2 concentration
Deoxynucleotide triphosphate
PH solution
2
1
2
PROTOCOLS
PCR Protocol
Step 1
Step 2
Etc.
DNA Measurement and Analysis Protocol
Step 1
Step 2
Etc.
New System: Research and Development
BACKGROUND
DESIGN
Primers for PCR
Our primers address the following design needs
Design specification 1 - explanation of how an aspect of the primers addresses any of the specifications in the "New System: Design Strategy" section
Design specification 2 - explanation of how an aspect of the primers addresses any of the specifications in the "New System: Design Strategy" section
Etc.
New System: Software
[THIS SECTION IS OPTIONAL. If your team has creative ideas for new software, and new software is a key component included in your new protocols, R&D, or machine design, you may describe it here. You will not receive bonus points, but a solid effort may raise your overall page layout points. If you decide not to propose new software, please delete this entire section, including the ==New System: Software== header.]