BME103 s2013:T900 Group9: Difference between revisions
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| [[ | | [[Image:BME103 Group9ColeyWhite Assembly.jpg|100px|thumb|Name: Coley White<br>Role(s): Protocol Planner]] | ||
| [[Image: | | [[Image:BME103 Group3 AimenVanood.jpg|100px|thumb|Name: Aimen Vanood<br>Role(s): Research and development]] | ||
| [[Image:Nordy.gif|100px|thumb|Name: Brady Falk<br>Role(s): Machine testing]] | | [[Image:Nordy.gif|100px|thumb|Name: Brady Falk<br>Role(s): Machine testing]] | ||
| [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | | [[Image:BME103student.jpg|100px|thumb|Name: student<br>Role(s)]] | ||
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| <p>Positive control:</p> <p>cancer DNA template</p> Tube label: '''+''' || <p>Patient 1</p> <p> ID: '''10840'''</p> <p>Replicate 1</p> <p> Tube label '''A1'''</p> || <p>Patient 1</p> <p> ID: '''10840'''</p> <p>Replicate 2</p> <p> Tube label ''' | | <p>Positive control:</p> <p>cancer DNA template</p> Tube label: '''+''' || <p>Patient 1</p> <p> ID: '''10840'''</p> <p>Replicate 1</p> <p> Tube label '''A1'''</p> || <p>Patient 1</p> <p> ID: '''10840'''</p> <p>Replicate 2</p> <p> Tube label '''A2'''</p> || <p>Patient 1</p> <p> ID: ['''10840'''</p> <p>Replicate 3</p> <p> Tube label '''A3'''</p> | ||
|- | |- | ||
| <p>Negative control:</p> <p>cancer DNA template</p> Tube label: '''-''' || <p>Patient 2</p> <p> ID: '''12675'''</p> <p>Replicate 1</p> <p> Tube label '''B1'''</p> || <p>Patient 2</p> <p> ID: '''12675'''</p> <p>Replicate 2</p> <p> Tube label '''B2'''</p> || <p>Patient 2</p> <p> ID: '''12675'''</p> <p>Replicate 3</p> <p> Tube label '''B3''' | | <p>Negative control:</p> <p>cancer DNA template</p> Tube label: '''-''' || <p>Patient 2</p> <p> ID: '''12675'''</p> <p>Replicate 1</p> <p> Tube label '''B1'''</p> || <p>Patient 2</p> <p> ID: '''12675'''</p> <p>Replicate 2</p> <p> Tube label '''B2'''</p> || <p>Patient 2</p> <p> ID: '''12675'''</p> <p>Replicate 3</p> <p> Tube label '''B3''' |
Latest revision as of 11:19, 2 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | ||||||||||||||
OUR TEAMLAB 1 WRITE-UPInitial Machine TestingThe Original Design
When we unplugged the Liquid Crystal Display from the open PCR circuit board, the machine no longer displayed onything on the LCD screen. When we unplugged the white wire that connects the circuit board to 16-tube PCR block, the machine would not produce any heat through the 16-tube PCR block. Anything connected to the circuit board, such as the LCD display or the 16-tube PCR block, will not run when the connection is disrupted.
The first test was run on March 5, 2013 using Machine 13. The sample of DNA was put into the PCR machine and all of the results came out as expected. There were no problems while testing.
ProtocolsThermal Cycler Program Heated Lid: 110°C Initial Step: temp: 95°C time: 180 sec Number of Cycles: 35 Denaturing: temp: 95°C time: 30 sec Annealing: temp: 57°C time: 30 sec Extending: temp: 72°C time: 30 sec Final Hold: temp: 4°C After the thermal cycler program is set up, we then proceeded with the experiment by saving the settings and selecting the "Plug in OpenPCR to Start" option. DNA Sample Set-up
DNA Sample Set-up Procedure
PCR Reaction Mix The mix is composed of:* Taq DNA polymerase * MgCl2 * dNTP’s. DNA/ primer mix The DNA/ primer mix is composed of: * Either one of the DNA of patient 1 or patient 2. * A forward and reverse primer, which will be the same and present in all tubes.
Research and DevelopmentSpecific Cancer Marker Detection - The Underlying Technology (Add a write-up of the information discussed in Week 3's class) (BONUS points: Use a program like Powerpoint, Word, Illustrator, Microsoft Paint, etc. to illustrate how primers bind to the cancer DNA template, and how Taq polymerases amplify the DNA. Screen-captures from the OpenPCR tutorial might be useful. Be sure to credit the source if you borrow images.)
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