SYBR Green Dye [A short summary describing SYBR green dye]
Single-Drop Fluorimeter [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
[The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash: None
ISO setting: 800
White Balance: Auto
Exposure: +2
Saturation: +2
Contrast:-2
[If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash:N/A
ISO setting:N/A
White Balance: N/A
Exposure:N/A
Saturation:N/A
Contrast:N/A
Calibration
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
Distance between the smart phone cradle and drop = 3.7 cm
[See worksheet page 5]
Solutions Used for Calibration[See worksheet page 5]Image J Values for All Samples[See worksheet page 5]
Calf Thymus
DNA solution
concentration
(microg/mL)
Volume of the
2X DNA
solution
(µL)
Volume of the
SYBR GREEN I
Dye solution
(µL)
Final DNA
concentration in
PicoGreen Assay
(ng/mL)
5
80
80
2.5
2
80
80
1
1
80
80
0.5
0.5
80
80
0.25
0.25
80
80
0.125
0
80
80
blank
[Add more rows as needed]
Placing Samples onto the Fluorimeter
[Step one, in your OWN words]
[Step two, in your own words]
[Step three, in your own words]
[etc.]
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]