BME103 s2013:T900 Group9 L2: Difference between revisions
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[[Image:sdp.png|400px|]]<br> | [[Image:sdp.png|400px|]]<br> | ||
(image used from google images)<br> | |||
'''SYBR Green Dye'''<br> | '''SYBR Green Dye'''<br> | ||
''SYBR Green Dye is a type of dye that is used to highlight when dsDNA is present because that is when it fluoresces the strongest. The dye fluoresces weak with water or single strands of DNA. | ''SYBR Green Dye is a type of dye that is used to highlight when dsDNA is present because that is when it fluoresces the strongest. The dye fluoresces weak with water or single strands of DNA. ''<br> | ||
'''Single-Drop Fluorimeter'''<br> | '''Single-Drop Fluorimeter'''<br> | ||
''The Single-Drop Fluorimeter is a custom device designed for the purpose of finding when a double-stranded piece of DNA is present. It fluoresces double-stranded DNA fragments in a green color, and does nothing to a solution without the double-stranded piece of DNA. [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br> | ''The Single-Drop Fluorimeter (as pictured above)is a custom device designed for the purpose of finding when a double-stranded piece of DNA is present. It fluoresces double-stranded DNA fragments in a green color, and does nothing to a solution without the double-stranded piece of DNA. [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]''<br> | ||
'''How the Fluorescence Technique Works'''<br> | '''How the Fluorescence Technique Works'''<br> | ||
'The process had several different variables that were chosen for very specific reasons. The slide to be used was chosen because it had a rough surface with glass circles exposed in it, causing the solution to concentrate at those locations and be easier to examine. The DNA dye chosen for the experiment was used because it was not completely soluble, which allowed for the light to glow instead of causing a reflective light off of the drop.' | |||
Revision as of 18:25, 1 April 2013
BME 103 Spring 2013 | Home People Lab Write-Up 1 Lab Write-Up 2 Lab Write-Up 3 Course Logistics For Instructors Photos Wiki Editing Help | |||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||||
OUR TEAM
LAB 2 WRITE-UPBackground Information
SYBR Green Dye
ProcedureSmart Phone Camera Settings
Calibration [Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
[See worksheet page 5]
Placing Samples onto the Fluorimeter
Data AnalysisRepresentative Images of Samples Sample with DNA (positive signal)
Fitting a Straight Line
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