SYBR Green Dye [A short summary describing SYBR green dye]
Single-Drop Fluorimeter [A description of the single-drop fluorimeter device. Add a PHOTO for bonus points]
How the Fluorescence Technique Works [In your own words, a summary of the information from page 9 of the worksheet]
Procedure
Smart Phone Camera Settings
[The type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash: None
ISO setting: 800
White Balance: Auto
Exposure: +2
Saturation: +2
Contrast:-2
[If you used an additional phone, describe the other type of smart phone you used and how you adjusted the camera settings, if applicable (see worksheet page 4).]
Flash:N/A
ISO setting:N/A
White Balance: N/A
Exposure:N/A
Saturation:N/A
Contrast:N/A
Calibration
[Describe how to set up your camera in front of the fluorimeter. Add a PHOTO of this set-up for bonus points.]
Distance between the smart phone cradle and drop = 3.7 cm
[See worksheet page 5]
Solutions Used for Calibration[See worksheet page 5]Image J Values for All Samples[See worksheet page 5]
Calf Thymus
DNA solution
concentration
(microg/mL)
Volume of the
2X DNA
solution
(µL)
Volume of the
SYBR GREEN I
Dye solution
(µL)
Final DNA
concentration in
PicoGreen Assay
(ng/mL)
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
row 2 cell 5
row 3 cell 1
row 3 cell 2
row 3 cell 3
row 3 cell 4
row 2 cell 5
row 1 cell 1
row 1 cell 2
row 1 cell 3
row 1 cell 4
row 1 cell 5
row 2 cell 1
row 2 cell 2
row 2 cell 3
row 2 cell 4
row 2 cell 5
row 3 cell 1
row 3 cell 2
row 3 cell 3
row 3 cell 4
row 2 cell 5
row 3 cell 1
row 3 cell 2
row 3 cell 3
row 3 cell 4
row 2 cell 5
[Add more rows as needed]
Placing Samples onto the Fluorimeter
[Step one, in your OWN words]
[Step two, in your own words]
[Step three, in your own words]
[etc.]
Data Analysis
Representative Images of Samples
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with no DNA]
[Show an IMAGE where you drew a circle around the droplet with the freehand tool for a sample with DNA (positive signal)]
Calf Thymus DNA
concentration (FINAL),
migrog/mL
Area
Mean Pixel Value
RAWINTDEN
of the drop
RAWINTDEN
of the background
Water
40636
32.437
1318101
302462
0.25
39770
64.565
2567761
243302
0.25
43440
48.047
2087153
50662
0.25
38994
82.158
3203667
239145
0.5
45724
105.339
4770813
286980
0.5
37690
83.846
3160168
251141
0.5
38724
73.079
2829899
54829
1.00
41200
105.656
4353036
288678
1.00
43584
113.023
4926005
335726
1.00
41404
105.037
4348994
279375
2.00
40024
135.205
5411434
326434
2.00
40968
103.250
4229944
260408
2.00
40111
129.871
5209254
425061
5.00
40950
132.782
5437418
393246
5.00
40468
147.876
5984250
352233
5.00
39796
140.267
5582067
341520
[Add more rows as needed]
Fitting a Straight Line [Place an IMAGE of your Excel plot with a line of best fit here. See worksheet page 8]