BME494:Materials: Difference between revisions
From OpenWetWare
Jump to navigationJump to search
No edit summary |
|||
Line 29: | Line 29: | ||
'''PROCEDURE'''<br> | '''PROCEDURE'''<br> | ||
* ''E. coli'' (NEB10B) ells were grown overnight in selective LB liquid medium. For the | * ''E. coli'' (NEB10B) ells were grown overnight in selective LB liquid medium. For the class demonstration experiment, Dr. Haynes induced a Lac-repressed GFP with varying amounts of IPTG. | ||
* 1 mL samples were transferred from large liquid cultures into tapered plastic cuvettes. | * 1 mL samples were transferred from large liquid cultures into tapered plastic cuvettes. | ||
* The spectral analyzer probe was placed against the clear wall of the cuvette. | * The spectral analyzer probe was placed against the clear wall of the cuvette. | ||
Line 37: | Line 37: | ||
'''Dr. Vikram Kodibagkar''' is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the spectral analyzer. | '''Dr. Vikram Kodibagkar''' is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the spectral analyzer. | ||
==Fluorescence Imaging Web Cam== | ==Fluorescence Imaging Web Cam== |
Revision as of 16:07, 6 March 2012
Home People Course Projects Course Materials Syllabus Photos Wiki Editing Help
Spectral Analyzer: Live GFP MeasurementsWe used an in-house built spectral analyzer to measure GFP fluorescence of liquid E. coli cultures. A "pen light" probe was placed against the wall of a cuvette that held a sample of liquid cell culture. The pen light served as the excitation light source, and also detected emission coming back from the sample, in this case, 520 nm emission from GFP. PROCEDURE
Dr. Vikram Kodibagkar is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the spectral analyzer. Fluorescence Imaging Web CamDetails coming soon |