BME494:Materials - Revision history

Karmella Haynes: /* Fluorescence Imaging Web Cam */

Karmella Haynes — Tue, 06 Mar 2012 23:21:34 GMT

Fluorescence Imaging Web Cam

←Older revision		Revision as of 23:21, 6 March 2012
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	<br>		<br>

-	'''Dr. Vikram Kodibagkar''' is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the ~~spectral analyzer~~.	+	'''Dr. Vikram Kodibagkar''' is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the fluorescence imaging web cam.

Karmella Haynes — Tue, 06 Mar 2012 23:21:01 GMT

Fluorescence Imaging Web Cam

←Older revision		Revision as of 23:21, 6 March 2012
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	Details coming soon		Details coming soon
		+
		+	<br>
		+
		+	'''Dr. Vikram Kodibagkar''' is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the spectral analyzer.
		+

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Karmella Haynes — Tue, 06 Mar 2012 23:07:16 GMT

Spectral Analyzer: Live GFP Measurements

←Older revision		Revision as of 23:07, 6 March 2012
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	'''PROCEDURE'''<br>		'''PROCEDURE'''<br>
-	* ''E. coli'' (NEB10B) ells were grown overnight in selective LB liquid medium. For the ~~course~~ demonstration experiment, Dr. Haynes induced a Lac-repressed GFP with varying amounts of IPTG.	+	* ''E. coli'' (NEB10B) ells were grown overnight in selective LB liquid medium. For the class demonstration experiment, Dr. Haynes induced a Lac-repressed GFP with varying amounts of IPTG.
	* 1 mL samples were transferred from large liquid cultures into tapered plastic cuvettes.		* 1 mL samples were transferred from large liquid cultures into tapered plastic cuvettes.
	* The spectral analyzer probe was placed against the clear wall of the cuvette.		* The spectral analyzer probe was placed against the clear wall of the cuvette.
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	'''Dr. Vikram Kodibagkar''' is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the spectral analyzer.		'''Dr. Vikram Kodibagkar''' is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the spectral analyzer.
-

	==Fluorescence Imaging Web Cam==		==Fluorescence Imaging Web Cam==

Karmella Haynes — Tue, 06 Mar 2012 23:05:03 GMT

←Older revision		Revision as of 23:05, 6 March 2012
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-	~~Content~~ coming soon	+	==Spectral Analyzer: Live GFP Measurements==
		+
		+	We used an in-house built spectral analyzer to measure GFP fluorescence of liquid ''E. coli'' cultures. A "pen light" probe was placed against the wall of a cuvette that held a sample of liquid cell culture. The pen light served as the excitation light source, and also detected emission coming back from the sample, in this case, 520 nm emission from GFP.<br><br>
		+
		+	'''PROCEDURE'''<br>
		+	* ''E. coli'' (NEB10B) ells were grown overnight in selective LB liquid medium. For the course demonstration experiment, Dr. Haynes induced a Lac-repressed GFP with varying amounts of IPTG.
		+	* 1 mL samples were transferred from large liquid cultures into tapered plastic cuvettes.
		+	* The spectral analyzer probe was placed against the clear wall of the cuvette.
		+	* The analyzer displayed the entire emission spectrum of the sample in real time.
		+	* The peak height (y) at 520 nm (x) was recorded for each experimental sample, one positive control, and one negative control.
		+	<br>
		+
		+	'''Dr. Vikram Kodibagkar''' is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the spectral analyzer.
		+
		+
		+	==Fluorescence Imaging Web Cam==
		+
		+	Details coming soon

	\|}		\|}

Karmella Haynes — Mon, 05 Mar 2012 03:36:21 GMT

←Older revision		Revision as of 03:36, 5 March 2012
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