Spectral Analyzer: Live GFP Measurements
We used an in-house built spectral analyzer to measure GFP fluorescence of liquid E. coli cultures. A "pen light" probe was placed against the wall of a cuvette that held a sample of liquid cell culture. The pen light served as the excitation light source, and also detected emission coming back from the sample, in this case, 520 nm emission from GFP.
Dr. Vikram Kodibagkar is a professor in the Biomedical Engineering program who studies biological imaging. He designed and generously provided the spectral analyzer.
Fluorescence Imaging Web Cam
Details coming soon