BME494 Project Group4

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ABSTRACT File:BME494 placeholder Write a description of the image here BACKGROUND File:BME494 placeholder Write a description of the image here A toggle switch is an important design motif in synthetic biology as it allows for the use of different constructs dependent on the active chemicals and gene sequences involved. This allows for multiple constructs to be activated at different times dependent on the wants of the synthetic biologist who created the BioBrick. Expression levels of different gene sequences can be controlled by various conditions such as the presence of ligand molecules in culture media. We improved upon the genetic toggle switch by designing a toggle mechanism that is initiated in the presence of two different wavelengths of light (green and blue light). This allows us to control the rate of reaction of these constructs simply by imploring specific wavelengths that are read by a gene sequence sensitive to that wavelength. The control of this construct is given to the individual performing the experiment as they can induce a gene of interest by simply shining a specific wavelength of blue light onto the construct. In the case of this period of the process, the sequence simply glows red when it is being induced, however, this can be changed at any time to induce nearly any gene of interest. When the construct is completed and all data is collected, the sequence can be terminated with the use of a green light which induces specific aspects of the construct to be activated, and eliminating previous processes. PROOF OF CONCEPT DESIGN New Natural Part: Why are you using this part? How did you find this part? What database/ resources did you use? What primers will you use to isolate it and turn it into a BioBrick? Key Pre-existing Part: If you didn't need to use a new natural part, describe one important pre-existing BioBrick from the parts registry.org that you will use. Why are you using it? Describe how well-documented the part is. Do you trust it? Assembly Scheme File:BME494 placeholder TESTING Measurement Expected Observations File:BME494 placehlder.jpg Tuning Our System File:BME494 placeolder.jpg HUMAN PRACTICES OUR TEAM Your Name Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page. Your Name Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page. Your Name Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page. Your Name Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page.