BME494 Project Group5: Difference between revisions
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In this study we looked at the possibility of making an ''E. coli'' strain that has a plasmid which will | |||
allow for the production of gonadotropin-releasing hormone (GnRH) to help increase the level of | |||
testosterone. Low levels of testosterone stimulate the production of GnRH which indirectly stimulates | |||
the production of more testosterone via lutenizing hormone and follicle stimulating hormone. To show | |||
proof-of-concept, two plasmids were constructed working as a repressilator to have isolated production | |||
of both GnRH and the reporter molecule green fluorescent protein (GFP). Theoritically, having one | |||
plasmid create GnRH and the repressor for the GFP plasmid and the other plasmid create GFP and | |||
repressor for the GnRH plasmid will allow for isolated production of both to show expression characteristics | |||
of GnRH being produced. Further testing must be done to address correct protein folding and activity. | |||
Using this proof-of-concept, it is hoped to develop a human-viable chassis to help those affected | |||
with hypotestosteronism. However, human practices involving genetically modified organisms to | |||
be “implanted” or given to humans still remains subject to debate in the scientific community. | |||
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Revision as of 10:03, 7 March 2012
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ABSTRACTIn this study we looked at the possibility of making an E. coli strain that has a plasmid which will allow for the production of gonadotropin-releasing hormone (GnRH) to help increase the level of testosterone. Low levels of testosterone stimulate the production of GnRH which indirectly stimulates the production of more testosterone via lutenizing hormone and follicle stimulating hormone. To show proof-of-concept, two plasmids were constructed working as a repressilator to have isolated production of both GnRH and the reporter molecule green fluorescent protein (GFP). Theoritically, having one plasmid create GnRH and the repressor for the GFP plasmid and the other plasmid create GFP and repressor for the GnRH plasmid will allow for isolated production of both to show expression characteristics of GnRH being produced. Further testing must be done to address correct protein folding and activity. Using this proof-of-concept, it is hoped to develop a human-viable chassis to help those affected with hypotestosteronism. However, human practices involving genetically modified organisms to be “implanted” or given to humans still remains subject to debate in the scientific community.
BACKGROUND
PROOF OF CONCEPT DESIGN
TESTING
HUMAN PRACTICESOUR TEAM
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