BME494 Project Group6: Difference between revisions
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==ABSTRACT== | ==ABSTRACT== | ||
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[[Image: | [[Image:479px-Cobalamin.png|thumb|frame|left|Vitamin B-12 (Cobalamin)]] | ||
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Revision as of 15:07, 10 March 2012
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ABSTRACTOur goal was to create a strain of E. Coli which could produce vitamin B-12 (cobalamin) to help people who have vitamin B-12 deficiency. Vitamin B-12 deficiency is especially common in vegetarians and vegans, because it is found almost exclusively in meats and dairy products. In order to achieve B-12 production in E. Coli, we extracted the DNA sequence coding for the cobW gene in Cupriavidus metallidurans using PCR, and inserted the gene into an Ampicillin resistant plasmid containing GFP for proof of concept. Although humans are fairly good at removing excess vitamin B-12 due to its solubility in water, having unrestrained B-12 production in the body still is an unnecessary risk. In order to control the production of B-12, we linked the promoter's activation to the presence of the amino acid homocysteine, which is an indicator of low B-12 levels. If successful, we will hopefully create a safe way to help those who suffer from vitamin B-12 deficiency to enjoy a better quality of life.
BACKGROUND
PROOF OF CONCEPT DESIGN
TESTING
HUMAN PRACTICESOUR TEAM
Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page.
Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page.
Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page.
Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page.
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