BME494 Project Group8

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ABSTRACT A ball-model of a ethanol molecule Our body has two enzymes that allow us to breakdown alcohol ADH (Ethanol – CH3CHO) and ALDH (CH3CHO -- CH3COOH). Our idea is to put these enzymes into Lactobacillus, a bacteria common in the intestinal track. Our modified bacteria can be put into yogurt and be administered by eating the yogurt. This will be more healthy then the drugs currently on the market for this purpose. Also these bacteria can stay in the gut for long periods of time increasing the treatment time. Our proof of concept will have a visual indicator to indicate how it is working, this is crucial for tuning. The first portion of our system is the control aspect and is a promoter that is repressed in the presence of ethanol and produces Green Florescent Protein (GFP). The second part of the system is induced in the presence of ethanol and produces the two proteins needed to break down ethanol and Red Florescent Protein (RFP), creating a visual indication of the production of the proteins. BACKGROUND File:BME494 placeholder Write a description of the image here Alcohol, more specifically ethanol is the most abused drug in the United States [1]. There have been many ways that people have tried to help alcoholics with their problem, and one way is medical intervention. The theory behind our design is that if the alcohol is broken-down before it gets into the blood stream you can stop the person from getting drunk and stop the cycle of alcohol abuse. PROOF OF CONCEPT DESIGN New Natural Part: Why are you using this part? How did you find this part? What database/ resources did you use? What primers will you use to isolate it and turn it into a BioBrick? Key Pre-existing Part: If you didn't need to use a new natural part, describe one important pre-existing BioBrick from the parts registry.org that you will use. Why are you using it? Describe how well-documented the part is. Do you trust it? Assembly Scheme File:BME494 placeholder TESTING Measurement Expected Observations Tuning Our System HUMAN PRACTICES OUR TEAM Your Name Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page. Your Name Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page. Your Name Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page. Your Name Your area of study/ academic program/ major, why you are taking BME494, and something interesting about yourself. You may add a link to your personal OWW page.