BME494 Project Group9
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ABSTRACT
BACKGROUND
The proposed design has the potential to be used as a simple and efficient diagnostic test.
PROOF OF CONCEPT DESIGNIn the presence of 25(OH)D3 in blood, the co-transcription factor and promoter for the CYP21B1 are activated. When the promoter is activated, it expresses the enzyme that causes a chemical change to to the active form of Vitamin D. This active form, 1-25-DiHydroxyVitamin D3, is created in the kidney. If the promoter is activated, then it will express the enzyme that chemically changes to the active form of vitamin D in the kidney.
FUTURE VISION: D-Vitameter: Ideally this would be a device that would benefit from the proposed technology. This compact and portable device would allow patients to get a reading of their ability to produce Vitamin D by just using one drop of blood. The basic components of this small device would be an enclosed box with a small polycarbonate window. The device will allow to introduce a sample in a microscope slide and shine a UV light to detect presence of 25(OH).
TESTING
When 25(OH)D3 is present, ideally there would be a 100% GFP concentration
HUMAN PRACTICES•The meter is practical because the blood sample is relatively small and can be extracted at any time. Normal tests for Vitamin D deficiency require laboratory procedures and time.
OUR TEAM
Biomedical Engineering, SBHSE, I am interested in learning the general concepts of synthetic biology and its applications, I will be graduating this semester! http://openwetware.org/wiki/User:Carolina_Tostado
Biochemistry.
Biomedical Engineering, SBHSE.
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