Overview & Purpose
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Design: Our genetic circuit
OUR GENE SWITCH:
Device design. Image adapted from
Building: Assembly Scheme
Testing: Modeling and GFP Imaging
A LAC SWITCH MODEL
We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.
AN INTERACTIVE MODEL
We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.
COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL
We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.
Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.
Danger of Chemicals in Farmlands
- My name is Shay Ravacchioli, and I am a Junior majoring in Biomedical Engineering with minors in Biological Sciences and Psychology. I am taking BME 494 because I think Synthetic Biology is fascinating. An interesting fact about me is that I play piano and guitar.
- My name is Michael Rose, and I am a Senior majoring in BME. I am taking BME 494 because . An interesting fact about me is that ###.
- My name is ###, and I am a ### majoring in ###. I am taking BME 494 because I find synthetic biology very interesting and I want to learn more about it. An interesting fact about me is that I love to cook.
- My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.
 Heller, H. Craig., David M. Hillis, Gordon H. Orians, William K. Purves, and David Sadava. Life: The Science of Biology. Sunderland, MA,: Sinauer Ass., W.H. Freeman and, 2008. N. pag. Print.
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