BME494s2013 Project Team2

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[[Image:Photo_(29).jpg |thumb|noframe|130px| Degree=90|left|'''Michael Rose''']]  
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Revision as of 19:16, 24 April 2013


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Contents

Overview & Purpose

Text describing the image
Text describing the image












Background

Wow the Lac Operon
Wow the Lac Operon


Wow the Lac Operon
Wow the Lac Operon













Design: Our genetic circuit

OUR GENE SWITCH:


Device design. Image adapted from
Device design. Image adapted from












Building: Assembly Scheme













Testing: Modeling and GFP Imaging


A LAC SWITCH MODEL
We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated.


AN INTERACTIVE MODEL
We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system.


COLLECTING IMPERICAL VALUES TO IMPROVE THE MODEL
We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions.



Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model.











Human Practices

Danger of Chemicals in Farmlands
Danger of Chemicals in Farmlands














Our Team

Shay Ravacchioli
Shay Ravacchioli


  • My name is Shay Ravacchioli, and I am a Junior majoring in Biomedical Engineering with minors in Biological Sciences and Psychology. I am taking BME 494 because I think Synthetic Biology is fascinating. An interesting fact about me is that I play piano and guitar.



Jenessa Lancaster
Jenessa Lancaster


  • My name is Jenessa Lancaster, and I am a Junior majoring in Biomedical Engineering with a minor in Psychology. I am taking BME 494 because I have always wanted to learn more about Synthetic Biology and Genetic Engineering. An interesting fact about me is that I write songs.



Michael Rose
Michael Rose


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.



Your Name
Your Name


  • My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###.






Works Cited

[1] Heller, H. Craig., David M. Hillis, Gordon H. Orians, William K. Purves, and David Sadava. Life: The Science of Biology. Sunderland, MA,: Sinauer Ass., W.H. Freeman and, 2008. N. pag. Print.

[2] Full reference.

[3] Full reference.


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