BME494s2013 Project Template: Difference between revisions
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== | ==Design: Our genetic circuit== | ||
<!--Delete the line items that do not apply to your project --> | <!--Delete the line items that do not apply to your project --> | ||
'''OUR GENE SWITCH''': | '''OUR GENE SWITCH''': | ||
<!-- Show a network/ circuit diagram of your team's Lac switch. Include a paragraph to explain how it works --> | |||
<!-- Show a network/ circuit diagram of your team's Lac switch. Include a paragraph to explain how it works (i.e., how to switch the system from on to off and vice versa, and what happens to each component as the system switches between states) --> | |||
[[Image:CirEmblemHandwriteLogo2.png|300px|Text describing the image]] | [[Image:CirEmblemHandwriteLogo2.png|300px|Text describing the image]] | ||
<br><br><br><br><br><br><br><br><br> | |||
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==Building: Assembly Scheme== | |||
<!-- Illustrate and describe how you will build your lac switch. Incorporate information from Group Presentation 2 --> | |||
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==Testing: Modeling and GFP Imaging== | ==Testing: Modeling and GFP Imaging== | ||
<br> | <br> | ||
''' | '''A LAC SWITCH MODEL''' | ||
<br> | <br> | ||
<!-- | We used a previously published synthetic switch, developed by Ceroni et al., to understand how our system could potentially be modeled and simulated. | ||
<!-- Continue this paragraph by explaining to a non-specialist what a mathematical model is and what parameter values are, in general. Include your network diagram illustration of the Ceroni et al. model and list all of the parameters you were able to map onto the model --> | |||
'''AN INTERACTIVE MODEL''' | |||
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''' | We used a model of the natural Lac operon to understand how changing the parameter values changes the behavior of the system. | ||
<!-- Continue this paragraph by explaining how you interacted with the MatLab model. Include two or more images showing different output curves that were generated when you altered the IPTG concentration --> | |||
'''COLLECTING EMPIRICAL VALUES TO IMPROVE THE MODEL''' | |||
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<!-- | We explored how one technique, imaging via microscopy could be used to determine the production rate of an output protein, in this case GFP in yeast, could be used to determine a "real" value for maximum GFP production rate under our own laboratory conditions. | ||
<!-- Describe summarize how you measured GFP intensities, plotted charts in MatLab, make a curve of best fit, and tried to determine the maximum rate of GFP production. You don't have to include your raw values, just the graphs and equations. You may include some small images of the GFP-expressing yeast. If you didn't get a nice peak, explain how your image analysis might be changed to improve your outcome. --> | |||
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Ideally, the GFP production rate measured by this method could be entered as a value for [which parameter] in the Ceroni et al. model. | |||
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==Human Practices== | ==Human Practices== | ||
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<!--Wait until Unit 3 to fill this in. Demonstrate that you have considered the societal aspects of your project - what could go wrong? Is it implementable? Etc.--> | <!--Wait until Unit 3 to fill this in. Demonstrate that you have considered the societal aspects of your project - what could go wrong? Is it implementable? Etc.--> | ||
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==Our Team== | ==Our Team== | ||
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<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px"> | <div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px"> | ||
[[Image: | [[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']] | ||
* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###. | |||
*My name is | |||
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<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px"> | <div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px"> | ||
[[Image: | [[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']] | ||
* | * My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###. | ||
</div> | |||
<br> | <br> | ||
<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px"> | <div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px"> | ||
[[Image: | [[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']] | ||
* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###. | |||
</div> | |||
<br> | |||
<div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px"> | <div style="color: #808080; background-color: #ffffff; width: 600px; padding: 5px"> | ||
[[Image: | [[Image:BME194_profile.jpg |thumb|noframe|130px|left|'''Your Name''']] | ||
* My name is ###, and I am a ### majoring in ###. I am taking BME 494 because ###. An interesting fact about me is that ###. | |||
</div> | |||
<br> | |||
<br><br><br> | <br><br><br> | ||
==Works Cited== | ==Works Cited== | ||
[1] | [1] Full reference. | ||
[2] | [2] Full reference. | ||
[3] | [3] Full reference. | ||
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Latest revision as of 15:36, 22 April 2013
Home People Course Projects Course Materials Schedule Photos Wiki Editing Help
Overview & Purpose
Background
Design: Our genetic circuitOUR GENE SWITCH:
Building: Assembly Scheme
Testing: Modeling and GFP Imaging
Human Practices
Our Team
Works Cited[1] Full reference. [2] Full reference. [3] Full reference.
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