BM 506/2011:Schedule

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
Current revision (22:33, 7 October 2011) (view source)
(→Bahan Ketrampilan Dasar Ilmu Biomedik)
 
(13 intermediate revisions not shown.)
Line 3: Line 3:
==Jadwal Kuliah dan Praktikum Ketrampilan Dasar Laboratorium 2011==
==Jadwal Kuliah dan Praktikum Ketrampilan Dasar Laboratorium 2011==
<BR>
<BR>
 +
Lihat jadwal praktikum  [http://openwetware.org/images/c/c5/Jadwal_Praktikum_Biomedik_2011.pdf di sini.]
-
{| border="2"
+
==Bahan Ketrampilan Dasar Ilmu Biomedik==
-
|+
+
Bahan praktikum maupan kuliah bisa Anda dapat [http://openwetware.org/wiki/Magister_Ilmu_Biomedik:Notebook/Ketrampilan_Dasar_2011 di sini.]
-
! Monday    !! Tuesday    !! Wednesday !! Thursday  !! Friday 
+
-
|-
+
-
| August 29 <br>
+
-
| August 30 <br>'''Lab 1'''<br>Classes Start || August 31  <BR> '''Lab 1''' || September 1  <br> '''Lab 1''' || September 2 <br>'''Lab 1''' ||
+
-
|-
+
-
| September 5 <br>'''Labor Day <BR> NO CLASSES'''
+
-
| September 6 <br>'''Lab 2'''|| September 7 <br>''' Monday Schedule'''<BR>'''Lab 1''' || September 8<br>'''Lab 2'''|| September 9 <br>'''Lab 2''' || 
+
-
|-
+
-
| September 12 <br>'''Lab 2'''
+
-
| September 13 <br>'''Lab 3''' || September 14 <br>'''Lab 2''' || September 15 <br>'''NO LAB''' || September 16 <br>'''Lab 3''' || 
+
-
|-
+
-
| September 19 <br>'''Lab 3'''
+
-
| September 20 <br>'''Lab 4'''<br> || September 21 <br>'''Lab 3''' ||  September 22 <br>'''Lab 3''' || September 23 <br>'''Lab 4'''  || 
+
-
|-
+
-
| September 26 <br>'''Lab 4'''
+
-
| September 27 <br>'''Lab 5''' || September 28 <br>'''Lab 4''' ||  September 29 <br>'''Lab 4''' || September 30 <br>'''Lab 5''' || 
+
-
|-
+
-
| October 3 <br>'''Lab 5'''
+
-
| October 4 <br>'''Lab 6''' || October 5 <br> '''Lab 5'''  ||  October 6 <br> '''Lab 5'''|| October 7 <br>'''NO LAB''' || 
+
-
|-
+
-
| October 10 <br>'''FALL BREAK <BR> No Classes'''
+
-
| October 11 <br>'''FALL BREAK <BR> No Classes''' || October 12 <br>'''Lab 6''' ||  October 13 <br>'''Lab 6''' || October 14 <br>'''Lab 6'''|| 
+
-
|-
+
-
| October 17 <br>'''Lab 6'''
+
-
| October 18 <br>'''Lab 7''' || October 19 <br>'''Lab 7'''|| October 20 <br>'''Lab 7''' || October 21<BR>'''Lab 7'''|| 
+
-
|-
+
-
| October 24 <br>'''Lab 7'''
+
-
| October 25 <br> '''Lab 8''' || October 26 <br> '''Lab 8''' || October 27 <br> '''Lab 8'''|| October 28 <br> '''Lab 8'''|| 
+
-
|-
+
-
| October 31 <br> '''Lab 8'''
+
-
| November 1 <br>'''Tanner Conference <BR> No Classes''' || November 2  <br>'''Lab 9''' || November 3 <br>'''Lab 9''' || November 4 <br>'''Lab 9'''|| 
+
-
|-
+
-
| November 7<br> '''Lab 9'''
+
-
| November 8 <br>'''Lab 9''' || November 9  <br>'''Lab 10''' || November 10 <br>'''Lab 10''' || November 11 <br>'''Lab 10'''|| 
+
-
|-
+
-
| November 14<br> '''Lab 10'''
+
-
| November 15 <br>'''Lab 10''' || November 16  <br>'''Lab 11''' || November 17 <br>'''Lab 11''' || November 18 <br>'''Lab 11'''|| 
+
-
|-
+
-
| November 21 <br> '''NO LAB <br> Photosynthesis paper due'''<br>
+
-
| November 22 <br>'''NO LAB'''<br>'''Photosynthesis paper due''' || November 23  <br>'''Thanksgiving Break<BR> No Classes''' || November 24 <br>'''Thanksgiving Break <BR> No Classes'''|| November 25 <br> '''Thanksgiving Break <BR> No Classes'''|| 
+
-
|-
+
-
| November 28 <br>'''Lab 11'''
+
-
| November 29 <br>'''Lab 11''' || November 30 <br>'''Lab 12'''  || December 1 <br>'''Lab 12''' || December 2 <br>'''Lab 12'''|| 
+
-
|-
+
-
| December 5 <br> '''Lab 12'''
+
-
| December 6 <br> '''Lab 12''' || December 7  <br>'''Last Day of Classes<BR> NO LAB''' || December 8 || December 9 || 
+
-
|-
+
-
|}
+
-
<br>
+
-
=='''Schedule of Experiments'''==
+
Bahan kuliah dan materi tugas kuliah lainnya bisa didapat [http://openwetware.org/wiki/User:Donny_Nauphar/Notebook/Program_Magister_Biomedik_FK_USU di sini.]
-
{| border="1"
+
-
|+
+
-
! Section !! Lab # !! Activities
+
-
|-
+
-
! 1
+
-
| 1-5 || Boot Camp: Metric Measurement;<BR> Using Basic Lab Equipment;<BR> Making Solutions and Dilutions;<BR> Microscopy; Scientific Investigation;<BR> Designing & Executing Experiments;<BR> Data Analysis & Display; <BR>Statistical parameters; Scientific Writing
+
-
|-
+
-
!2
+
-
| 6-8
+
-
|Genetics: DNA extraction, Visualization by agarose gel electrophoresis;<BR> Genotype/phenotype via Taster SNP data collection and analysis;<BR> Scientific Writing & Effective figure design
+
-
|-
+
-
!3
+
-
|8-10
+
-
|Photosynthesis: Spectrophotometry: Beer-Lambert Law, Linear regression analysis;<BR>  Hill Reaction: Self-designed investigation of factors <BR>affecting photosystems
+
-
|-
+
-
!4
+
-
|11
+
-
|Gene Regulation/Enzymology: Invertase enzyme assay
+
-
|-
+
-
!Lab Practical
+
-
|12
+
-
|Hands-on skills assessment:
+
-
|-
+
-
|}
+
-
<br>
+
-
 
+
-
== '''BISC110/112 Assignments/ Weekly Lab Planner''' ==
+
-
 
+
-
{| border=1px
+
-
|'''Series'''
+
-
|'''Lab #'''
+
-
|'''LAB DATES'''
+
-
|'''LAB DESCRIPTION'''
+
-
|'''Assignment/Notes'''
+
-
|--
+
-
| colspan="6" |
+
-
|--
+
-
| 1
+
-
| 1
+
-
| Aug.30 to<br> Sept. 7
+
-
| Practice metric conversions; <BR>Practice pipetting; <BR>Making Solutions; Microscopy; <BR>Examination of ''Tetrahymena pyriformis''<br>
+
-
| '''Homework''': Familiarize yourself with the wiki, particularly the information in the [[BISC110/F11:Resources]] section of the Lab wiki; <BR>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_Problems110.doc]];<br>
+
-
Read about the process of endocytosis in your textbook;<BR> familiarize yourself with phagocytosis in ''Tetrahymena''<BR> from the following reference articles:<BR> '''*Gronlien HK, Berg T, Lovlie AM.''' In the polymorphic ciliate ''Tetrahymena vorax'',<BR> the non-selective phagocytosis seen in microstomes <BR>changes to a highly selective process in macrostomes.<BR> (2002), Journal of Experimental Biology 205, 2089-2097; <BR>
+
-
'''*McLaughlin NB and Buhse HE, Jr.,''' Localization by indirect immunofluorescence of tetrin, actin,<BR> and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257.
+
-
|--
+
-
| 1
+
-
| 2
+
-
| Sept. 6 to<br> Sept. 14
+
-
| ''Tetrahymena'' Behavior: Phagocytosis; Observations; Data Analysis;<BR> Accuracy vs. Precision; Statistical Parameters;<BR> Data Presentation; Effective Figure Design and Legends<BR> using the Gronlien article as a model.
+
-
| '''Homework''': Study dilution making for Lab 3; <BR>Generate two figures with legends from your ''Tetrahymena'' experiment:<BR> one or more microphotographs of Tetrahymena and a graph or table of data.
+
-
|--
+
-
| 1
+
-
| 3
+
-
| Sept. 13 to<br> Sept. 19
+
-
| Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena'';<BR> Design your own experiment exploring<BR> inhibitors and ''Tetrahymena'' phagocytosis
+
-
| '''Homework''': There are animated tutorials on dilution<BR> available at the following link:<BR> [http://www.wellesley.edu/Biology/Concepts/Html/dilutions.html Dilution Help Tutorials]; <br> Finalize your experimental design for Lab 4; have it approved by your lab instructor;<br> Write a results section of a scientific report<BR> on your findings in the ''Tetrahymena'' toxicity experiment <BR>including a narrative portion and integrated figure(s) with legend(s);<br>
+
-
Solve the dilution problems found at<BR> [[Media: Lab_3_110_Dilution_Problems.doc]] and hand them in<BR> by the beginning of Lab 4.
+
-
|--
+
-
| 1
+
-
| 4
+
-
| Sept. 20 to <br> Sept. 29
+
-
| Student Designed Experiments:<BR>Potential Inhibitors of Vacuole Formation;
+
-
| '''Homework''': 5 minute oral presentation in PowerPoint™ of your data,<BR> including at least one figure with legend, of your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion <BR>'''* Gronlien, H.K., Berg, T., Lovlie, A.M.''' (2002).<BR> In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes<BR> to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097
+
-
|--
+
-
| 1
+
-
| 5
+
-
| Sept.27 to <br> Oct. 6
+
-
| Scientific Writing Workshop with Journal Article Discussion
+
-
| '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/F11:Resources | Resources]]; <BR>Science Writing peer-tutor appointment scheduling at: <BR>[http://www.wellesley.edu/Writing/Program/tutors.html Wellesley College Writing Program Resources].<br>
+
-
Have Lab notebooks ready to be turned in for inspection after Lab 6.
+
-
<br> Read Lab 6 (first lab in Series 2, Genetics)
+
-
<br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures.
+
-
|--
+
-
| 2
+
-
| 6
+
-
| Oct.  12 to<br> Oct. 17
+
-
| Genetics: Taster SNP 1-Test PTC Taster Phenotype;<BR> Isolate and digest DNA; PCR;
+
-
| '''Homework''': Read the following background information on<BR> [http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf restriction enzymes] <BR>and on [http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf gel electrophoresis] <br>
+
-
Read the following background information on ethical issues in genetic testing:<br>
+
-
'''[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710700/?tool=pubmed Direct-to-Consumer Genetic Testing,'''<BR> Two Sides of the Coin] by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18. <BR>
+
-
[http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf PUBLIC HEALTH: <BR>A Case Study of Personalized Medicine] by S. H. Katsanis, G. Javitt, and K. Hudson,<BR> published in ''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum)<BR>
+
-
[http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 AMA DTC testing policy recommendations] <br>
+
-
Draw the expected migration locations of the Fnu4H1 digested PCR product<BR> obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; <br>
+
-
Answer the questions found at:<BR>[[Media:Taster_DTC_questions110.doc‎]] <BR>to use as a template for your answers.'''
+
-
|--
+
-
| colspan="6" |
+
-
|--
+
-
| 2
+
-
| 7
+
-
| Oct. 18 to <br> Oct. 24
+
-
| Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis;<BR> Determine PTC Taster Genotype; Scientific Writing: <BR>Introduction & Discussion
+
-
| '''Homework:
+
-
Prepare draft figures/tables from the preliminary course data<BR> for the taster investigation paper and an skeleton outline<BR> of your introduction & discussion sections.<BR> (Refer to the handout on How to Prepare a Skeleton Outline in Sakai.)
+
-
|--
+
-
| 2 & 3
+
-
| 8
+
-
| Oct. 25 to<br> Oct. 31
+
-
| Data Analysis of Taster investigation; Science Writing Workshop:<BR> Effective Figure Design, Introduction, Discussion; Photosynthesis 1:<BR> Chlorophyll pigment investigation, Spectroscopy,<BR> The Beer-Lambert Law and Creating a Standard Curve
+
-
| '''Homework''': Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.'''<BR> For help, see the [[BISC110/F11:Resources | Resources]] section of this wiki.<br>
+
-
In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br>
+
-
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br>
+
-
|--
+
-
| 3
+
-
| 9
+
-
| Nov. 2 to<br> Nov. 8
+
-
| Photosynthesis 2: Hill Reaction
+
-
| '''Homework''': Finalize your self-designed experiment<BR> testing a variable affecting the Hill Reaction;<br> Write a Materials and Methods style version of the Hill Reaction<BR> experiment that you did this week in Lab 9
+
-
|--
+
-
| 3
+
-
| 10
+
-
| Nov. 9 to <br> Nov. 15
+
-
| Photosynthesis 3: Investigation of variables affecting the Hill Reaction
+
-
| '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis)<BR> for the different treatments (with the treatments on the same graph).<BR>See([[Appendix_E/F11 |Appendix E]]). <BR> Determine regression equations for the linear part of each data plots<BR> to get slope (m) values (slopes represent the rates of electron transport). <BR>Calculate the rates (change over time of the linear portion of your graphs)<BR> and plot those values against your variable (x-axis);<br>
+
-
'''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11 (or on day indicated in Lab Calendar).'''<BR>  Discuss your results in terms of how the factor that you chose to investigate<BR> affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/F11:Guidelines for Science Writing | '''Guidelines for science writing''']]" information<BR> found in the [[BISC110/F11:Resources | Resources]] section of the wiki.
+
-
|--
+
-
| 4
+
-
| 11
+
-
| Nov. 16 to<br> Nov. 29
+
-
| Enzymology<BR>Answer the following questions (5 points) <BR>and hand them in to your instructor before you leave lab. <BR>Download a Word document form of these questions here:[[Media: Lab_11_Questions_110_112.doc]]
+
-
| '''Homework: Prepare for a Lab Practical Skills Assessment in lab 12.'''<br>
+
-
 
+
-
<br> Make sure Lab Notebook is ready to turn in for grading at the beginning of LAB 12
+
-
|--
+
-
| Skills Assessment
+
-
| 12
+
-
| Nov. 30 to <br> Dec. 6
+
-
| '''Lab Practical'''; <br>Turn in Lab Notebook
+
-
| End of lab
+
-
|--
+
-
|}
+
-
<br>
+
-
 
+
-
=='''Lab Graded Assignments'''==
+
-
{| border="1"
+
-
|+
+
-
! Lab Assigned !! Lab Due !! Assignment<br> description !! Point Value <br>(Total 200)
+
-
|-
+
-
! 1
+
-
| 2
+
-
| Practice Problems
+
-
| 5
+
-
|-
+
-
! 2
+
-
| 3
+
-
| Fig. Design
+
-
| 5
+
-
|-
+
-
! 3
+
-
| 4
+
-
| Dilution Problems (5);<br>Fig.& Results (10)
+
-
| 15
+
-
|-
+
-
! 4
+
-
| 5
+
-
| Oral presentation: Results, original figure & legend;<br> Journal article discussion
+
-
| 10
+
-
|-
+
-
! 5
+
-
| 6
+
-
| Lab notebook check (ungraded);<br> Partial Scientific Paper(25)
+
-
| 25
+
-
|-
+
-
! 6
+
-
| 7
+
-
| SNP Taster Questions
+
-
| 5
+
-
|-
+
-
! 7
+
-
| 8
+
-
| Draft figures/tables & outline of Taster paper
+
-
| 5
+
-
|-
+
-
! 8
+
-
| 9
+
-
| Taster Scientific Paper (all but M&M)
+
-
| 35
+
-
|-
+
-
! 9
+
-
| 10
+
-
| M&M (5pt);<br> Finalize Self-Design <br>Experiment Hill Rxn.(ungraded)
+
-
| 5
+
-
|-
+
-
! 10
+
-
| 11 (or on day indicated in calendar)
+
-
| Full Scientific Paper: Factor Affecting Rate of Photosynthesis
+
-
| 35
+
-
|-
+
-
! 11
+
-
| 11 (In Lab)
+
-
| Concept Questions Enzymology/Gene Reg.
+
-
| 5
+
-
|-
+
-
! 12
+
-
|12
+
-
|Lab Notebook Check (10) <BR>Lab Practical:Hands-on skills assessment (35)<br>
+
-
| 45
+
-
|-
+
-
! Other
+
-
| -
+
-
| Discretionary Points <br> Preparation & Participation
+
-
| 5
+
-
|-
+
-
|}
+
-
<br>
+
-
 
+
-
</div>
+

Current revision

Ilmu Biomedik FK USU BM 506/2011

Home        Kita        Jadwal        Help        Campus USU       

Jadwal Kuliah dan Praktikum Ketrampilan Dasar Laboratorium 2011


Lihat jadwal praktikum di sini.

Bahan Ketrampilan Dasar Ilmu Biomedik

Bahan praktikum maupan kuliah bisa Anda dapat di sini.

Bahan kuliah dan materi tugas kuliah lainnya bisa didapat di sini.

Retrieved from "http://www.openwetware.org/wiki/BM_506/2011:Schedule"
Personal tools