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(New page: {{Template:BISC110/F11}} <div style="padding: 10px; width: 725px; border: 5px solid #397D02;"> ==BISC110/112 Fall 2011 Lab Calendar== <BR> {| border="2" |+ ! Monday !! Tuesday !!...)
 
 
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==BISC110/112 Fall 2011 Lab Calendar==
==Jadwal Kuliah dan Praktikum Ketrampilan Dasar Laboratorium 2011==
<BR>
<BR>
Lihat jadwal praktikum  [http://openwetware.org/images/c/c5/Jadwal_Praktikum_Biomedik_2011.pdf di sini.]


{| border="2"
==Bahan Ketrampilan Dasar Ilmu Biomedik==
|+
Bahan praktikum maupan kuliah bisa Anda dapat [http://openwetware.org/wiki/Magister_Ilmu_Biomedik:Notebook/Ketrampilan_Dasar_2011 di sini.]
! Monday    !! Tuesday    !! Wednesday !! Thursday  !! Friday 
|-
| August 29 <br>
| August 30 <br>'''Lab 1'''<br>Classes Start || August 31  <BR> '''Lab 1''' || September 1  <br> '''Lab 1''' || September 2 <br>'''Lab 1''' ||
|-
| September 5 <br>'''Labor Day <BR> NO CLASSES'''
| September 6 <br>'''Lab 2'''|| September 7 <br>''' Monday Schedule'''<BR>'''Lab 1''' || September 8<br>'''Lab 2'''|| September 9 <br>'''Lab 2''' || 
|-
| September 12 <br>'''Lab 2'''
| September 13 <br>'''Lab 3''' || September 14 <br>'''Lab 2''' || September 15 <br>'''NO LAB''' || September 16 <br>'''Lab 3''' || 
|-
| September 19 <br>'''Lab 3'''
| September 20 <br>'''Lab 4'''<br> || September 21 <br>'''Lab 3''' ||  September 22 <br>'''Lab 3''' || September 23 <br>'''Lab 4'''  || 
|-
| September 26 <br>'''Lab 4'''
| September 27 <br>'''Lab 5''' || September 28 <br>'''Lab 4''' ||  September 29 <br>'''Lab 4''' || September 30 <br>'''Lab 5''' || 
|-
| October 3 <br>'''Lab 5'''
| October 4 <br>'''Lab 6''' || October 5 <br> '''Lab 5'''  ||  October 6 <br> '''Lab 5'''|| October 7 <br>'''NO LAB''' || 
|-
| October 10 <br>'''FALL BREAK <BR> No Classes'''
| October 11 <br>'''FALL BREAK <BR> No Classes''' || October 12 <br>'''Lab 6''' ||  October 13 <br>'''Lab 6''' || October 14 <br>'''Lab 6'''|| 
|-
| October 17 <br>'''Lab 6'''
| October 18 <br>'''Lab 7''' || October 19 <br>'''Lab 7'''|| October 20 <br>'''Lab 7''' || October 21<BR>'''Lab 7'''|| 
|-
| October 24 <br>'''Lab 7'''
| October 25 <br> '''Lab 8''' || October 26 <br> '''Lab 8''' || October 27 <br> '''Lab 8'''|| October 28 <br> '''Lab 8'''|| 
|-
| October 31 <br> '''Lab 8'''
| November 1 <br>'''Tanner Conference <BR> No Classes''' || November 2  <br>'''Lab 9''' || November 3 <br>'''Lab 9''' || November 4 <br>'''Lab 9'''|| 
|-
| November 7<br> '''Lab 9'''
| November 8 <br>'''Lab 9''' || November 9  <br>'''Lab 10''' || November 10 <br>'''Lab 10''' || November 11 <br>'''Lab 10'''|| 
|-
| November 14<br> '''Lab 10'''
| November 15 <br>'''Lab 10''' || November 16  <br>'''Lab 11''' || November 17 <br>'''Lab 11''' || November 18 <br>'''Lab 11'''|| 
|-
| November 21 <br> '''NO LAB <br> Photosynthesis paper due'''<br>
| November 22 <br>'''NO LAB'''<br>'''Photosynthesis paper due''' || November 23  <br>'''Thanksgiving Break<BR> No Classes''' || November 24 <br>'''Thanksgiving Break <BR> No Classes'''|| November 25 <br> '''Thanksgiving Break <BR> No Classes'''|| 
|-
| November 28 <br>'''Lab 11'''
| November 29 <br>'''Lab 11''' || November 30 <br>'''Lab 12'''  || December 1 <br>'''Lab 12''' || December 2 <br>'''Lab 12'''|| 
|-
| December 5 <br> '''Lab 12'''
| December 6 <br> '''Lab 12''' || December 7  <br>'''Last Day of Classes<BR> NO LAB''' || December 8 || December 9 || 
|-
|}
<br>


=='''Schedule of Experiments'''==
Bahan kuliah dan materi tugas kuliah lainnya bisa didapat [http://openwetware.org/wiki/User:Donny_Nauphar/Notebook/Program_Magister_Biomedik_FK_USU di sini.]
{| border="1"
|+
! Section !! Lab # !! Activities
|-
! 1
| 1-5 || Boot Camp: Metric Measurement;<BR> Using Basic Lab Equipment;<BR> Making Solutions and Dilutions;<BR> Microscopy; Scientific Investigation;<BR> Designing & Executing Experiments;<BR> Data Analysis & Display; <BR>Statistical parameters; Scientific Writing
|-
!2
| 6-8
|Genetics: DNA extraction, Visualization by agarose gel electrophoresis;<BR> Genotype/phenotype via Taster SNP data collection and analysis;<BR> Scientific Writing & Effective figure design
|-
!3
|8-10
|Photosynthesis: Spectrophotometry: Beer-Lambert Law, Linear regression analysis;<BR>  Hill Reaction: Self-designed investigation of factors <BR>affecting photosystems
|-
!4
|11
|Gene Regulation/Enzymology: Invertase enzyme assay
|-
!Lab Practical
|12
|Hands-on skills assessment:
|-
|}
<br>
 
== '''BISC110/112 Assignments/ Weekly Lab Planner''' ==
 
{| border=1px
|'''Series'''
|'''Lab #'''
|'''LAB DATES'''
|'''LAB DESCRIPTION'''
|'''Assignment/Notes'''
|--
| colspan="6" |
|--
| 1
| 1
| Aug.30 to<br> Sept. 7
| Practice metric conversions; <BR>Practice pipetting; <BR>Making Solutions; Microscopy; <BR>Examination of ''Tetrahymena pyriformis''<br>
| '''Homework''': Familiarize yourself with the wiki, particularly the information in the [[BISC110/F11:Resources]] section of the Lab wiki; <BR>Lab 1 Practice Problems downloadable here [[Media:Lab_1_Practice_Problems110.doc]];<br>
Read about the process of endocytosis in your textbook;<BR> familiarize yourself with phagocytosis in ''Tetrahymena''<BR> from the following reference articles:<BR> '''*Gronlien HK, Berg T, Lovlie AM.''' In the polymorphic ciliate ''Tetrahymena vorax'',<BR> the non-selective phagocytosis seen in microstomes <BR>changes to a highly selective process in macrostomes.<BR> (2002), Journal of Experimental Biology 205, 2089-2097; <BR>
'''*McLaughlin NB and Buhse HE, Jr.,''' Localization by indirect immunofluorescence of tetrin, actin,<BR> and centrin to the oral apparatus and buccal cavity of the macrostomal form of ''Tetrahymena vorax''., (2004), J Eukaryot. Microbiol., 51(2), 253-257.
|--
| 1
| 2
| Sept. 6 to<br> Sept. 14
| ''Tetrahymena'' Behavior: Phagocytosis; Observations; Data Analysis;<BR> Accuracy vs. Precision; Statistical Parameters;<BR> Data Presentation; Effective Figure Design and Legends<BR> using the Gronlien article as a model.
| '''Homework''': Study dilution making for Lab 3; <BR>Generate two figures with legends from your ''Tetrahymena'' experiment:<BR> one or more microphotographs of Tetrahymena and a graph or table of data.
|--
| 1
| 3
| Sept. 13 to<br> Sept. 19
| Making Dilutions; Determination of toxicity of Heavy Metals on ''Tetrahymena'';<BR> Design your own experiment exploring<BR> inhibitors and ''Tetrahymena'' phagocytosis
| '''Homework''': There are animated tutorials on dilution<BR> available at the following link:<BR> [http://www.wellesley.edu/Biology/Concepts/Html/dilutions.html Dilution Help Tutorials]; <br> Finalize your experimental design for Lab 4; have it approved by your lab instructor;<br> Write a results section of a scientific report<BR> on your findings in the ''Tetrahymena'' toxicity experiment <BR>including a narrative portion and integrated figure(s) with legend(s);<br>
Solve the dilution problems found at<BR> [[Media: Lab_3_110_Dilution_Problems.doc]] and hand them in<BR> by the beginning of Lab 4.
|--
| 1
| 4
| Sept. 20 to <br> Sept. 29
| Student Designed Experiments:<BR>Potential Inhibitors of Vacuole Formation;
| '''Homework''': 5 minute oral presentation in PowerPoint™ of your data,<BR> including at least one figure with legend, of your Lab 4 experimental results. <br>Read assigned journal article for science writing discussion <BR>'''* Gronlien, H.K., Berg, T., Lovlie, A.M.''' (2002).<BR> In the polymorphic ciliate ''Tetrahymena vorax'', the non-selective phagocytosis seen in microstomes changes<BR> to a highly selective process in macrostomes, J. Exp. Biol. 205, 2089-2097
|--
| 1
| 5
| Sept.27 to <br> Oct. 6
| Scientific Writing Workshop with Journal Article Discussion
| '''Homework''': '''''Tetrahymena'' Partial Scientific Research Report: DUE AT THE BEGINNING OF LAB 6'''<BR> Title Page, Results, Discussion and References sections. <BR>Guidelines at:[[BISC110/F11:Resources | Resources]]; <BR>Science Writing peer-tutor appointment scheduling at: <BR>[http://www.wellesley.edu/Writing/Program/tutors.html Wellesley College Writing Program Resources].<br>
Have Lab notebooks ready to be turned in for inspection after Lab 6.
<br> Read Lab 6 (first lab in Series 2, Genetics)
<br>Prepare a flow chart in your lab notebook of all Lab 6 experimental procedures.
|--
| 2
| 6
| Oct.  12 to<br> Oct. 17
| Genetics: Taster SNP 1-Test PTC Taster Phenotype;<BR> Isolate and digest DNA; PCR;
| '''Homework''': Read the following background information on<BR> [http://serc.carleton.edu/files/genomics/units/snp_restriction_enzymes.pdf restriction enzymes] <BR>and on [http://serc.carleton.edu/files/genomics/units/snp_gel_background.pdf gel electrophoresis] <br>
Read the following background information on ethical issues in genetic testing:<br>
'''[http://www.ncbi.nlm.nih.gov/pmc/articles/PMC2710700/?tool=pubmed Direct-to-Consumer Genetic Testing,'''<BR> Two Sides of the Coin] by Farkas and Holland, J Mol Diagn. 2009 Jul;11(4):263-5. Epub 2009 Jun 18. <BR>
[http://0-www.sciencemag.org.luna.wellesley.edu/cgi/reprint/320/5872/53.pdf PUBLIC HEALTH: <BR>A Case Study of Personalized Medicine] by S. H. Katsanis, G. Javitt, and K. Hudson,<BR> published in ''Science'', 4 April 2008 320: 53-54 [DOI: 10.1126/science.1156604](in Policy Forum)<BR>
[http://www.dnapolicy.org/news.enews.article.nocategory.php?action=detail&newsletter_id=35&article_id=156 AMA DTC testing policy recommendations] <br>
Draw the expected migration locations of the Fnu4H1 digested PCR product<BR> obtained from a homozygous taster (left lane), a homozygous nontaster (center lane)<BR> and a heterozygote (right lane) on a diagram of an agarose gel; <br>
Answer the questions found at:<BR>[[Media:Taster_DTC_questions110.doc‎]] <BR>to use as a template for your answers.'''
|--
| colspan="6" |
|--
| 2
| 7
| Oct. 18 to <br> Oct. 24
| Genetics: Taster SNP 2: Prepare and perform agarose gel electrophoresis;<BR> Determine PTC Taster Genotype; Scientific Writing: <BR>Introduction & Discussion
| '''Homework:
Prepare draft figures/tables from the preliminary course data<BR> for the taster investigation paper and an skeleton outline<BR> of your introduction & discussion sections.<BR> (Refer to the handout on How to Prepare a Skeleton Outline in Sakai.)
|--
| 2 & 3
| 8
| Oct. 25 to<br> Oct. 31
| Data Analysis of Taster investigation; Science Writing Workshop:<BR> Effective Figure Design, Introduction, Discussion; Photosynthesis 1:<BR> Chlorophyll pigment investigation, Spectroscopy,<BR> The Beer-Lambert Law and Creating a Standard Curve
| '''Homework''': Write a scientific research report (all sections except M&M)on your PTC Taster SNP study.'''<BR> For help, see the [[BISC110/F11:Resources | Resources]] section of this wiki.<br>
In your lab notebook, prepare a flow chart for the Hill Reaction (Lab 9);<br>
Review the mechanisms of electron transport and proton flow in photosynthesis (use your textbook).<br>
|--
| 3
| 9
| Nov. 2 to<br> Nov. 8
| Photosynthesis 2: Hill Reaction
| '''Homework''': Finalize your self-designed experiment<BR> testing a variable affecting the Hill Reaction;<br> Write a Materials and Methods style version of the Hill Reaction<BR> experiment that you did this week in Lab 9
|--
| 3
| 10
| Nov. 9 to <br> Nov. 15
| Photosynthesis 3: Investigation of variables affecting the Hill Reaction
| '''Homework''': Plot your A580 data (y-axis) versus time in seconds (x-axis)<BR> for the different treatments (with the treatments on the same graph).<BR>See([[Appendix_E/F11 |Appendix E]]). <BR> Determine regression equations for the linear part of each data plots<BR> to get slope (m) values (slopes represent the rates of electron transport). <BR>Calculate the rates (change over time of the linear portion of your graphs)<BR> and plot those values against your variable (x-axis);<br>
'''A full paper on your experiment, in the form of a scientific research report,<BR> is due at beginning of Lab 11 (or on day indicated in Lab Calendar).'''<BR>  Discuss your results in terms of how the factor that you chose to investigate<BR> affects the electron transport rate in photosynthesis.<BR> Compare your results to other investigations published in the primary literature.<BR> Refer to the "[[BISC110/F11:Guidelines for Science Writing | '''Guidelines for science writing''']]" information<BR> found in the [[BISC110/F11:Resources | Resources]] section of the wiki.
|--
| 4
| 11
| Nov. 16 to<br> Nov. 29
| Enzymology<BR>Answer the following questions (5 points) <BR>and hand them in to your instructor before you leave lab. <BR>Download a Word document form of these questions here:[[Media: Lab_11_Questions_110_112.doc]]
| '''Homework: Prepare for a Lab Practical Skills Assessment in lab 12.'''<br>
 
<br> Make sure Lab Notebook is ready to turn in for grading at the beginning of LAB 12
|--
| Skills Assessment
| 12
| Nov. 30 to <br> Dec. 6
| '''Lab Practical'''; <br>Turn in Lab Notebook
| End of lab
|--
|}
<br>
 
=='''Lab Graded Assignments'''==
{| border="1"
|+
! Lab Assigned !! Lab Due !! Assignment<br> description !! Point Value <br>(Total 200)
|-
! 1
| 2
| Practice Problems
| 5
|-
! 2
| 3
| Fig. Design
| 5
|-
! 3
| 4
| Dilution Problems (5);<br>Fig.& Results (10)
| 15
|-
! 4
| 5
| Oral presentation: Results, original figure & legend;<br> Journal article discussion
| 10
|-
! 5
| 6
| Lab notebook check (ungraded);<br> Partial Scientific Paper(25)
| 25
|-
! 6
| 7
| SNP Taster Questions
| 5
|-
! 7
| 8
| Draft figures/tables & outline of Taster paper
| 5
|-
! 8
| 9
| Taster Scientific Paper (all but M&M)
| 35
|-
! 9
| 10
| M&M (5pt);<br> Finalize Self-Design <br>Experiment Hill Rxn.(ungraded)
| 5
|-
! 10
| 11 (or on day indicated in calendar)
| Full Scientific Paper: Factor Affecting Rate of Photosynthesis
| 35
|-
! 11
| 11 (In Lab)
| Concept Questions Enzymology/Gene Reg.
| 5
|-
! 12
|12
|Lab Notebook Check (10) <BR>Lab Practical:Hands-on skills assessment (35)<br>
| 45
|-
! Other
| -
| Discretionary Points <br> Preparation & Participation
| 5
|-
|}
<br>
 
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Latest revision as of 19:33, 7 October 2011

Ilmu Biomedik FK USU BM 506/2011

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Jadwal Kuliah dan Praktikum Ketrampilan Dasar Laboratorium 2011


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Bahan Ketrampilan Dasar Ilmu Biomedik

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Bahan kuliah dan materi tugas kuliah lainnya bisa didapat di sini.

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