BUGSS:Build-a-BUG:2-1: Difference between revisions
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* [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023457 One-Step Miniprep] | * [http://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0023457 One-Step Miniprep] | ||
* [http://openwetware.org/images/e/e3/EN-QuickLyse-Miniprep-Handbook.pdf QIAGEN QuickLyse Miniprep] | * [http://openwetware.org/images/e/e3/EN-QuickLyse-Miniprep-Handbook.pdf QIAGEN QuickLyse Miniprep] | ||
=== BioBrick parts === | |||
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K801000 BBa_K801000]: pTUM100 yeast shuttle vector based on pYES2 | |||
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K110006 BBa_K110006]: Alpha-Cell Promoter MF(ALPHA)6 | |||
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K110005 BBa_K110005]: Alpha-Cell Promoter MF(ALPHA)2 | |||
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K165057 BBa_K165057]: Kozak + mCherry translational unit | |||
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_K165059 BBa_K165059]: Kozak + CFPx2 translational unit | |||
* [http://partsregistry.org/wiki/index.php?title=Part:BBa_J63002 BBa_J63002]: ADH1 terminator from ''S. cerevisiae'' | |||
</div> | </div> | ||
Revision as of 20:45, 17 February 2013
Home Contact Internal Lab Members Projects Protocols Build-a-BUG Build-a-Gene 2016 Build-a-Gene 2015 Build-a-Gene 2014 Build-a-Gene 2013 Chalk Talks
Build-a-BUG: Series 2, Session 1
Saturday, February 9, 2013
Noon to 4:00 PM
This is the first Build-A-BUG workshop in a series of five on yeast. You will be introduced to basic lab techniques, including pipetting and centrifugation, and start your own mating type detector project. This lab will involve crash courses on molecular and cellular biology, the life cycle of budding yeast, and BioBrick-based approaches to synthetic biology, as well as hands-on minipreps (DNA isolation).
Background reading
- Life Cycle of the Budding Yeast Saccharomyces cerevisiae
- Sporulation in the Budding Yeast Saccharomyces cerevisiae
- BioBrick Assembly standard 10
Protocols
BioBrick parts
- BBa_K801000: pTUM100 yeast shuttle vector based on pYES2
- BBa_K110006: Alpha-Cell Promoter MF(ALPHA)6
- BBa_K110005: Alpha-Cell Promoter MF(ALPHA)2
- BBa_K165057: Kozak + mCherry translational unit
- BBa_K165059: Kozak + CFPx2 translational unit
- BBa_J63002: ADH1 terminator from S. cerevisiae
