BUGSS:Build-a-BUG:2-2

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''Noon to 4:00 PM''<br>
''Noon to 4:00 PM''<br>
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This is the second Build-A-BUG workshop in a series of five on yeast.  You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project.  This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and possibly ligations (linking DNA).<br>
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This is the second Build-A-BUG workshop in a series of five on yeast.  You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project.  This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and column-based DNA purifications.<br>
=== Background reading ===
=== Background reading ===
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=== Protocols ===
=== Protocols ===
* [https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions Optimizing Restriction Endonuclease Reactions]
* [https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions Optimizing Restriction Endonuclease Reactions]
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* [https://www.neb.com/protocols/1/01/01/dna-ligation-with-t4-dna-ligase-m0202 DNA ligation with T4 DNA Ligase]
 
* [[Agarose_gel_electrophoresis | Agarose gel electrophoresis]]
* [[Agarose_gel_electrophoresis | Agarose gel electrophoresis]]
* [http://openwetware.org/images/f/fe/QIAquick-Gel-Extraction-Kit-EN.pdf QIAquick Gel Extraction Protocol] and [http://openwetware.org/images/7/7f/EN-QIAquick-Spin-Handbook.pdf Handbook]
* [http://openwetware.org/images/f/fe/QIAquick-Gel-Extraction-Kit-EN.pdf QIAquick Gel Extraction Protocol] and [http://openwetware.org/images/7/7f/EN-QIAquick-Spin-Handbook.pdf Handbook]
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=== Restriction endonucleases & DNA ligase ===
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=== Restriction endonucleases ===
* [https://www.neb.com/products/r0101-ecori EcoRI]
* [https://www.neb.com/products/r0101-ecori EcoRI]
* [https://www.neb.com/products/r0145-xbai XbaI]
* [https://www.neb.com/products/r0145-xbai XbaI]
* [https://www.neb.com/products/r0133-spei SpeI]
* [https://www.neb.com/products/r0133-spei SpeI]
* [https://www.neb.com/products/r0140-psti PstI]
* [https://www.neb.com/products/r0140-psti PstI]
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* [https://www.neb.com/products/m0202-t4-dna-ligase T4 DNA Ligase]
 
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Revision as of 23:39, 3 March 2013

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Contents

Build-a-BUG: Series 2, Session 2

Saturday, February 23, 2013
Noon to 4:00 PM

This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and column-based DNA purifications.

Background reading

Protocols

Restriction endonucleases

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