BUGSS:Build-a-BUG:2-2: Difference between revisions
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''Noon to 4:00 PM''<br> | ''Noon to 4:00 PM''<br> | ||
This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and | This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and column-based DNA purifications.<br> | ||
=== Background reading === | === Background reading === | ||
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=== Protocols === | === Protocols === | ||
* [https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions Optimizing Restriction Endonuclease Reactions] | * [https://www.neb.com/protocols/2012/12/07/optimizing-restriction-endonuclease-reactions Optimizing Restriction Endonuclease Reactions] | ||
* [[Agarose_gel_electrophoresis | Agarose gel electrophoresis]] | * [[Agarose_gel_electrophoresis | Agarose gel electrophoresis]] | ||
* [http://openwetware.org/images/f/fe/QIAquick-Gel-Extraction-Kit-EN.pdf QIAquick Gel Extraction Protocol] and [http://openwetware.org/images/7/7f/EN-QIAquick-Spin-Handbook.pdf Handbook] | * [http://openwetware.org/images/f/fe/QIAquick-Gel-Extraction-Kit-EN.pdf QIAquick Gel Extraction Protocol] and [http://openwetware.org/images/7/7f/EN-QIAquick-Spin-Handbook.pdf Handbook] | ||
=== Restriction endonucleases | === Restriction endonucleases === | ||
* [https://www.neb.com/products/r0101-ecori EcoRI] | * [https://www.neb.com/products/r0101-ecori EcoRI] | ||
* [https://www.neb.com/products/r0145-xbai XbaI] | * [https://www.neb.com/products/r0145-xbai XbaI] | ||
* [https://www.neb.com/products/r0133-spei SpeI] | * [https://www.neb.com/products/r0133-spei SpeI] | ||
* [https://www.neb.com/products/r0140-psti PstI] | * [https://www.neb.com/products/r0140-psti PstI] | ||
</div> | </div> | ||
Revision as of 21:39, 3 March 2013
Home Contact Internal Lab Members Projects Protocols Build-a-BUG Build-a-Gene 2016 Build-a-Gene 2015 Build-a-Gene 2014 Build-a-Gene 2013 Chalk Talks
Build-a-BUG: Series 2, Session 2
Saturday, February 23, 2013
Noon to 4:00 PM
This is the second Build-A-BUG workshop in a series of five on yeast. You will continue to hone your basic lab techniques, including pipetting and centrifugation, while continuing either your own or somebody else's mating type detector project. This lab will involve restriction digests (cutting DNA), agarose gel electrophoresis (visualizing DNA), gel extractions (recovering DNA fragments), and column-based DNA purifications.
Background reading
Protocols
- Optimizing Restriction Endonuclease Reactions
- Agarose gel electrophoresis
- QIAquick Gel Extraction Protocol and Handbook
