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=== Background reading ===
=== Background reading ===
* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]] and [[BUGSS:Build-a-BUG:2-2 | Session 2]]
* Review [[BUGSS:Build-a-BUG:2-1 | Session 1]] and [[BUGSS:Build-a-BUG:2-2 | Session 2]]
* [http://www.ncbi.nlm.nih.gov/pmc/articles/PMC224649/pdf/pnas00115-0175.pdf Enzymatic breakage and joining of deoxyribonucleic acid, I. Repair of single-strand breaks in DNA by an enzyme system from Escherichia coli infected with T4 bacteriophage.]
* [http://www.ncbi.nlm.nih.gov/pubmed/4377758 DNA ligase: structure, mechanism, and function]
=== Protocols ===
=== Protocols ===

Revision as of 01:24, 4 March 2013


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Build-a-BUG: Series 2, Session 3

Saturday, March 9, 2013
Noon to 4:00 PM

This is the third Build-A-BUG workshop in a series of five on yeast. You will learn more about molecular cloning and continue to hone your basic lab techniques, while continuing either your own or somebody else's mating type detector project. This lab will involve ligations (linking DNA) and transformations of competent E. coli cells.

Background reading


DNA ligase

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