BUGSS:Build-a-Gene 2014: Difference between revisions
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In this 5-session “Build-a-Gene” class, we will learn and practice the techniques that synthetic biologists use in genome synthesis, including creation of synthetic genes, assembly of larger DNA fragments by Gibson Assembly, and the use of yeast cells for gene assembly. In addition to lab work, we will be discussing this brave new frontier in biological engineering- how this technology is currently being used to redesign cells and systems, and how society should respond to and regulate this new field. | In this 5-session “Build-a-Gene” class, we will learn and practice the techniques that synthetic biologists use in genome synthesis, including creation of synthetic genes, assembly of larger DNA fragments by Gibson Assembly, and the use of yeast cells for gene assembly. In addition to lab work, we will be discussing this brave new frontier in biological engineering- how this technology is currently being used to redesign cells and systems, and how society should respond to and regulate this new field. | ||
'''For additional reading on these topics, click on the links below.''' | |||
For an overview of synthetic genomics: [http://www.nature.com/nature/journal/v473/n7347/pdf/473403a.pdf] | |||
Synthetic Viruses: [http://mbio.asm.org/content/3/5/e00201-12.full.pdf][http://www.doublexscience.org/creating-viruses-to-create-the-vaccines-against-them/][http://labs.fhcrc.org/cbf/Papers/Viral_Library/0043_fauci.pdf][http://www.nytimes.com/2011/12/27/science/debate-persists-on-deadly-flu-made-airborne.html?pagewanted=all&_r=0] | |||
Synthetic Bacteria: [http://www.jcvi.org/cms/fileadmin/site/research/projects/first-self-replicating-bact-cell/fact-sheet2.pdf][http://www.jcvi.org/cms/fileadmin/site/research/projects/first-self-replicating-bact-cell/fact-sheet1.pdf][http://www.nature.com/news/2010/100524/full/news.2010.261.html][http://www.bu.edu/abl/files/nature_opinion.pdf] | |||
Synthetic Eukaryotes:[http://www.telegraph.co.uk/science/science-news/10171509/Synthetic-yeast-could-make-beer-cheaper-and-stronger.html] | |||
Future prospects: De-extinction and nightmare scenarios! [http://static.ow.ly/docs/dextinction_1fj6.pdf][http://ngm.nationalgeographic.com/2013/04/125-species-revival/zimmer-text][http://www.plosbiology.org/article/fetchObject.action?uri=info%3Adoi%2F10.1371%2Fjournal.pbio.1001530&representation=PDF] | |||
== Class sessions == | == Class sessions == | ||
Join us as we learn to synthesize new genes from scratch! The five sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all five sessions, each session can stand on its own. You do not need to commit to all five sessions to enjoy the experience. There are no prerequisites!<br> | |||
'''For detailed information and experimental protocols for each of the course sessions, click on the links below.''' | |||
'''Build-a-Gene 1:''' July 12, 2014. ''Gene Synthesis from Oligonucleotides''<br> | '''Build-a-Gene 1:''' July 12, 2014. ''Gene Synthesis from Oligonucleotides''<br> | ||
Revision as of 13:28, 6 July 2014
Class Overview
Since the Human Genome Project, scientists have become masters at reading the genetic code. But now, new technologies allow us to not only read DNA sequences, but also to write the genetic code! Scientists now regularly custom design and then synthesize short DNA sequence and are pioneering techniques to assemble larger custom genes and genomes. This unprecedented ability to create and manipulate living organisms has opened new frontiers in our ability to create life in the lab.
In this 5-session “Build-a-Gene” class, we will learn and practice the techniques that synthetic biologists use in genome synthesis, including creation of synthetic genes, assembly of larger DNA fragments by Gibson Assembly, and the use of yeast cells for gene assembly. In addition to lab work, we will be discussing this brave new frontier in biological engineering- how this technology is currently being used to redesign cells and systems, and how society should respond to and regulate this new field.
For additional reading on these topics, click on the links below.
For an overview of synthetic genomics: [1]
Synthetic Viruses: [2][3][4][5]
Synthetic Bacteria: [6][7][8][9]
Synthetic Eukaryotes:[10]
Future prospects: De-extinction and nightmare scenarios! [11][12][13]
Class sessions
Join us as we learn to synthesize new genes from scratch! The five sessions are designed to give a good survey of synthetic biology techniques while doing an interesting project. While we encourage you to take all five sessions, each session can stand on its own. You do not need to commit to all five sessions to enjoy the experience. There are no prerequisites!
For detailed information and experimental protocols for each of the course sessions, click on the links below.
Build-a-Gene 1: July 12, 2014. Gene Synthesis from Oligonucleotides
Build-a-Gene 2: July 26, 2014.
Build-a-Gene 3: August 9, 2014.
Build-a-Gene 4: August 23, 2014.
Build-a-Gene 5: August 30, 2014.
