BYU iGEM/Notebook/11 February 2011: Difference between revisions

Revision as of 14:42, 11 February 2011

We've designed our forward and reverse primers to clone out the ara-C gene and pBAD promoter for our thermoswitch plasmid.

ara-C pBAD Forward Primer:

gccAGATCTtaccgggtagaatcaaaccga

ara-C pBAD Reverse Primer: cggCTGCAGaaaaacgggTatggagaaaca

Redesigned the multiple cloning site (MCS) map in light of some new information regarding the OxyR binding site. See image below.

Revision as of 14:42, 11 February 2011 (view source) Chet Chamberlain (talk \| contribs) (→‎Thermoswitch Team) ← Older edit		Revision as of 14:42, 11 February 2011 (view source) Chet Chamberlain (talk \| contribs) (→‎Oxy Team) Newer edit →
Line 16:		Line 16:
	Redesigned the multiple cloning site (MCS) map in light of some new information regarding the OxyR binding site. See image below.		Redesigned the multiple cloning site (MCS) map in light of some new information regarding the OxyR binding site. See image below.

	[[Image:IMAG0070.jpg]]		[[Image:IMAG0070.jpg\|thumb\|left]]