BYU iGEM/Notebook/16 February 2011: Difference between revisions
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#Check PCR | #Check PCR | ||
*if good, restriction enzyme digest | #*if good, restriction enzyme digest | ||
*if not good, PCR again | #*if not good, PCR again | ||
#Plasmid: | |||
#*extract from E. coli | |||
*extract from E. coli | #*PCR amplification | ||
*PCR amplification | |||
==ΔTS Team To Do:== | ==ΔTS Team To Do:== | ||
Latest revision as of 16:37, 16 February 2011
OxySoxy Team To Do:
- Check PCR
- if good, restriction enzyme digest
- if not good, PCR again
- Plasmid:
- extract from E. coli
- PCR amplification
ΔTS Team To Do:
- PCR amplification of primers
- Plasmid extraction
- Insertion
