BYU iGEM/Notebook/16 February 2011: Difference between revisions
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==OxySoxy Team To Do:== | ==OxySoxy Team To Do:== | ||
Check PCR | #Check PCR | ||
*if good, restriction enzyme digest | *if good, restriction enzyme digest | ||
*if not good, PCR again | *if not good, PCR again | ||
Plasmid: | ##Plasmid: | ||
*extract from E. coli | *extract from E. coli | ||
*PCR amplification | *PCR amplification |
Revision as of 16:36, 16 February 2011
OxySoxy Team To Do:
- Check PCR
- if good, restriction enzyme digest
- if not good, PCR again
- Plasmid:
- extract from E. coli
- PCR amplification
ΔTS Team To Do:
- PCR amplification of primers
- Plasmid extraction
- Insertion