BYU iGEM/Notebook/31 January 2011: Difference between revisions
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(New page: =January 31 2011= ==Normal ROS levels in <i>E. coli</i>== Hydrogen peroxide: "The activity of alkylhydroperoxide reductase (Ahp) is so high that, although H2O2 is formed endogenously in...) |
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"Exponentially growing E. coli contains about 20 μM dimeric cytoplasmic SOD. Given that the rate of O2− formation is approximately 5 μM/s in these cells, the outcome is that the steady-state level of O2− is restricted to approximately 0.1 nM (53)." | "Exponentially growing E. coli contains about 20 μM dimeric cytoplasmic SOD. Given that the rate of O2− formation is approximately 5 μM/s in these cells, the outcome is that the steady-state level of O2− is restricted to approximately 0.1 nM (53)." | ||
Source: [http://www.annualreviews.org/doi/full/10.1146/annurev.biochem.77.061606.161055 | Source: [http://www.annualreviews.org/doi/full/10.1146/annurev.biochem.77.061606.161055 Cellular Defenses against Superoxide and Hydrogen Peroxide] | ||
Revision as of 15:29, 31 January 2011
January 31 2011
[Template:BYU iGEM]
Normal ROS levels in E. coli
Hydrogen peroxide:
"The activity of alkylhydroperoxide reductase (Ahp) is so high that, although H2O2 is formed endogenously inside aerobic E. coli at a rate of about 15 μM/s, the steady-state concentration does not exceed 20 nM."
Superoxide:
"Exponentially growing E. coli contains about 20 μM dimeric cytoplasmic SOD. Given that the rate of O2− formation is approximately 5 μM/s in these cells, the outcome is that the steady-state level of O2− is restricted to approximately 0.1 nM (53)."
Source: Cellular Defenses against Superoxide and Hydrogen Peroxide
