Baby cell column

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Material)
Line 9: Line 9:
! Part!! Company and cat. number!!Price!! Notes !! Link
! Part!! Company and cat. number!!Price!! Notes !! Link
|-
|-
-
| 1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLM, for 2,4mm tubing,  10 pices,||  Roth CT59.1|| 9.95 €|| ||http://www.carlroth.com
+
| 1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLM, for 2,4mm tubing,  10 pices||  Roth CT59.1|| 9.95 €|| ||http://www.carlroth.com
|-
|-
-
| 1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLF, for 2,4mm tubing,  10 pices || Roth CT63.1||  9.95 || ||
+
| 1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLF, for 2,4mm tubing,  10 pices || Roth CT63.1||  9.95 || ||
|-
|-
|1x Rotilabo®-Mini hose reducers, straight ,  2,4 – 4,8 mm,  10 pices||  Roth CT46.1||11.95 €  || ||
|1x Rotilabo®-Mini hose reducers, straight ,  2,4 – 4,8 mm,  10 pices||  Roth CT46.1||11.95 €  || ||
 +
|-
 +
|1x Rotilabo®-stoppers with hole, 23-29- 5mm hole, 10 pices || Roth Y852.1||18,90 € ||||
 +
|-
 +
|3x Rotilabo®-stand rods 600mm ||Roth 2378.1||8,95 € ||||
 +
|-
 +
|2x Rotilabo®-retort stand bases||Roth 2369.1||15.20 € ||||
 +
|-
 +
|7x Rotilabo®-cross double boss head||Roth 2194.1||7.95 € || ||
 +
|-
 +
|5x Rotilabo®-stand clamps, made of malleable iron, 40mm||Roth 1995.1||9.45 € ||||
 +
|-
 +
|1x Rotilabo®-tube clamps, Polysulfone (autoclavable)||Roth T764.1 ||32.80 € ||||
 +
|-
 +
|1x Corning® Erlenmeyer flask polycarbonate baffled flasks capacity 2000 mL, polypropylene cap (vented), package with 6 pices||Sigma CLS431256-6EA||300 €||||http://www.sigmaaldrich.com
 +
|-
 +
|
|}
|}
 +
==References==
==References==
<biblio>
<biblio>
#Bates2005MolMicro pmid=15978072
#Bates2005MolMicro pmid=15978072
</biblio>
</biblio>

Revision as of 05:36, 28 January 2009

The baby cell column is a way of getting synchronized bacterial cells (E. coli) which are at more or less the same point in their cell cycel.

Basic idea

Bacteria are attached to glass beads with there flagella. The protein coding for a specailly sticy flagella is introduced on the chromosome behind an inducible promotor. The glass beads with the cells are than put on a column and medium without inductor is run thru it. The new born cells will than not have a flagella and washed of with the medium. Such cells will be "babies" and at the beginning of the cell cycel.

Material

For the set up of the column one needs the following material

Part Company and cat. numberPrice Notes Link
1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLM, for 2,4mm tubing, 10 pices Roth CT59.1 9.95 € http://www.carlroth.com
1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLF, for 2,4mm tubing, 10 pices Roth CT63.1 9.95 €
1x Rotilabo®-Mini hose reducers, straight , 2,4 – 4,8 mm, 10 pices Roth CT46.111.95 €
1x Rotilabo®-stoppers with hole, 23-29- 5mm hole, 10 pices Roth Y852.118,90 €
3x Rotilabo®-stand rods 600mm Roth 2378.18,95 €
2x Rotilabo®-retort stand basesRoth 2369.115.20 €
7x Rotilabo®-cross double boss headRoth 2194.17.95 €
5x Rotilabo®-stand clamps, made of malleable iron, 40mmRoth 1995.19.45 €
1x Rotilabo®-tube clamps, Polysulfone (autoclavable)Roth T764.1 32.80 €
1x Corning® Erlenmeyer flask polycarbonate baffled flasks capacity 2000 mL, polypropylene cap (vented), package with 6 picesSigma CLS431256-6EA300 €http://www.sigmaaldrich.com

References

  1. Bates D, Epstein J, Boye E, Fahrner K, Berg H, and Kleckner N. . pmid:15978072. PubMed HubMed [Bates2005MolMicro]
Personal tools