Baby cell column: Difference between revisions
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(New page: The baby cell column is a way of getting synchronized bacterial cells (E. coli) which are at more or less the same point in their cell cycel. ==Basic idea== Bacteria are attached to glass...) |
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Bacteria are attached to glass beads with there flagella. The protein coding for a specailly sticy flagella is introduced on the chromosome behind an inducible promotor. The glass beads with the cells are than put on a column and medium without inductor is run thru it. The new born cells will than not have a flagella and washed of with the medium. Such cells will be "babies" and at the beginning of the cell cycel. | Bacteria are attached to glass beads with there flagella. The protein coding for a specailly sticy flagella is introduced on the chromosome behind an inducible promotor. The glass beads with the cells are than put on a column and medium without inductor is run thru it. The new born cells will than not have a flagella and washed of with the medium. Such cells will be "babies" and at the beginning of the cell cycel. | ||
==Material== | |||
For the set up of the column one needs the following material | |||
{| border="3" | |||
! Part!! Company and cat. number!!Price!! Notes !! Link | |||
|- | |||
| 1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLM, for 2,4mm tubing, 10 pices,|| Roth CT59.1|| 9.95 €|| ||http://www.carlroth.com | |||
|- | |||
| 1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLF, for 2,4mm tubing, 10 pices || Roth CT63.1|| 9.95 || || | |||
|- | |||
|1x Rotilabo®-Mini hose reducers, straight , 2,4 – 4,8 mm, 10 pices|| Roth CT46.1||11.95 € || || | |||
|} | |||
==References== | ==References== | ||
<biblio> | <biblio> | ||
#Bates2005MolMicro pmid=15978072 | #Bates2005MolMicro pmid=15978072 | ||
</biblio> | </biblio> | ||
Revision as of 01:54, 7 November 2008
The baby cell column is a way of getting synchronized bacterial cells (E. coli) which are at more or less the same point in their cell cycel.
Basic idea
Bacteria are attached to glass beads with there flagella. The protein coding for a specailly sticy flagella is introduced on the chromosome behind an inducible promotor. The glass beads with the cells are than put on a column and medium without inductor is run thru it. The new born cells will than not have a flagella and washed of with the medium. Such cells will be "babies" and at the beginning of the cell cycel.
Material
For the set up of the column one needs the following material
| Part | Company and cat. number | Price | Notes | Link |
|---|---|---|---|---|
| 1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLM, for 2,4mm tubing, 10 pices, | Roth CT59.1 | 9.95 € | http://www.carlroth.com | |
| 1x Rotilabo®-Luer-tubing connectors, Luer-fitting: LLF, for 2,4mm tubing, 10 pices | Roth CT63.1 | 9.95 | ||
| 1x Rotilabo®-Mini hose reducers, straight , 2,4 – 4,8 mm, 10 pices | Roth CT46.1 | 11.95 € |
