Bacterial species identification: Difference between revisions
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<cite> | |||
# pmid=1987160 | |||
# pmid=2095137 | |||
# pmid=14967233 | |||
# pmid=14607398 | |||
# pmid=11240048 | |||
# pmid=1987160 | |||
# pmid=2095137 | |||
# pmid=2482222 | |||
</cite> | |||
Mycoplasma universal primers | ==Mycoplasma universal primers== | ||
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998. | Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998. | ||
U1F 12-27 ttg atc ctg gct cag gay daa cg | * U1F 12-27 ttg atc ctg gct cag gay daa cg | ||
* U2F 327-348 cca rac tcc tac ggr agg c | |||
U2F 327-348 cca rac tcc tac ggr agg c | * U3R 512-527 att acc gcg gck gct g | ||
* U4F 792-810 gta gtc cac gcc gta aac g | |||
U3R 512-527 att acc gcg gck gct g | * U5R 902-924 ctt gtg cgg gyy ccc gtc aa | ||
* U6F 1154-1172 gag gaa ggy grg gay gay g | |||
U4F 792-810 gta gtc cac gcc gta aac g | * U7R 1359-1374 aca agr ccc gag aac g | ||
* U8R 1502-1524 gaa agg agg trw tcc ayc csc ac | |||
U5R 902-924 ctt gtg cgg gyy ccc gtc aa | |||
U6F 1154-1172 gag gaa ggy grg gay gay g | |||
U7R 1359-1374 aca agr ccc gag aac g | |||
U8R 1502-1524 gaa agg agg trw tcc ayc csc ac | |||
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175. | Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175. | ||
27f 5'AGAGTTTGATCMTGGCTCAG | * 27f 5'AGAGTTTGATCMTGGCTCAG | ||
342r 5'CTGCTGCSYCCCGTAG | * 342r 5'CTGCTGCSYCCCGTAG | ||
357f 5'CTCCTACGGGAGGCAGCAG | * 357f 5'CTCCTACGGGAGGCAGCAG | ||
519r 5'GWATTACCGCGGCKGCTG | * 519r 5'GWATTACCGCGGCKGCTG | ||
530f 5'GTGCCAGCMGCCGCGG | * 530f 5'GTGCCAGCMGCCGCGG | ||
1100r 5'GGGTTGCGCTCGTTG | * 1100r 5'GGGTTGCGCTCGTTG | ||
1114f 5'GCAACGAGCGCAACCC | * 1114f 5'GCAACGAGCGCAACCC | ||
1392r 5'ACGGGCGGTGTGTRC | * 1392r 5'ACGGGCGGTGTGTRC | ||
1406f 5'TGYACACACCTCCCGT | * 1406f 5'TGYACACACCTCCCGT | ||
1492r 5'TACGGYTACCTTGTTACGACTT | * 1492r 5'TACGGYTACCTTGTTACGACTT | ||
1525r 5'AAGGAGGTGWTCCARCC | * 1525r 5'AAGGAGGTGWTCCARCC | ||
Revision as of 18:50, 11 April 2006
[1, 1, 1, 1, 1, 1, 1, 1, 2, 2, 3, 3, 4, 5, 6, 7]
Mycoplasma universal primers
Johansson K-E, Characterization of mycoplasmas by PCR and sequence analysis iwth universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.
- U1F 12-27 ttg atc ctg gct cag gay daa cg
- U2F 327-348 cca rac tcc tac ggr agg c
- U3R 512-527 att acc gcg gck gct g
- U4F 792-810 gta gtc cac gcc gta aac g
- U5R 902-924 ctt gtg cgg gyy ccc gtc aa
- U6F 1154-1172 gag gaa ggy grg gay gay g
- U7R 1359-1374 aca agr ccc gag aac g
- U8R 1502-1524 gaa agg agg trw tcc ayc csc ac
Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.
- 27f 5'AGAGTTTGATCMTGGCTCAG
- 342r 5'CTGCTGCSYCCCGTAG
- 357f 5'CTCCTACGGGAGGCAGCAG
- 519r 5'GWATTACCGCGGCKGCTG
- 530f 5'GTGCCAGCMGCCGCGG
- 1100r 5'GGGTTGCGCTCGTTG
- 1114f 5'GCAACGAGCGCAACCC
- 1392r 5'ACGGGCGGTGTGTRC
- 1406f 5'TGYACACACCTCCCGT
- 1492r 5'TACGGYTACCTTGTTACGACTT
- 1525r 5'AAGGAGGTGWTCCARCC
