Bacterial transformation

From OpenWetWare

(Difference between revisions)
Jump to: navigation, search
(Chemical transformation)
Line 43: Line 43:
# Bloom05 US Patent 6,855,494 [[Media:pat6855494.pdf]]
# Bloom05 US Patent 6,855,494 [[Media:pat6855494.pdf]]
# Jessee05 US Patent 6,960,464 [[Media:pat6960464.pdf]]
# Jessee05 US Patent 6,960,464 [[Media:pat6960464.pdf]]
# Cohen-PNAS-1972 pmid=4559594
// This appears to be one of the earliest papers on transformation of Escherichia coli.  If you find an earlier paper, please include it here.

Revision as of 16:04, 20 December 2007

Back to Protocols



Transformation is the process of introducing foreign DNA (e.g plasmids, BAC) into a bacterium. Bacterial cells into which foreign DNA can be transformed are called competent. Some bacteria are naturally competent (e.g B. subtilis), whereas others such as E. coli are not naturally competent. Non-competent cells can be made competent and then transformed via one of two main approaches; chemical transformation and electroporation.

There are advantages and disadvantages to both transformation methods. In general, chemical transformation is less prone to error and faster however electroporation produces a higher transformation efficiency (fraction of transformed cells that actually uptake the foreign DNA). See Molecular Cloning for a fuller discussion of both approaches.


OpenWetWare already has a number of protocols relating to bacterial transformation but more are always welcome.

If you use a variant on one of these protocols please feel free to add a link to your protocol from one of these pages so other users can find a protocol that works for them. Additionally, if anyone uses the Innoe or Hanahan high-efficiency protocols, then please add protocols here.

Chemical transformation

If you plan on doing a chemical transformation, then you should see these pages -

Chemical transformation buffer comparison

Someone should check out the claims of Nishimura90. tk 08:58, 25 September 2007 (EDT)


If you plan on using electroporation, then see these pages -


Error fetching PMID 1943786:
Error fetching PMID 2235524:
Error fetching PMID 4559594:
  1. isbn:0-87969-577-3. [MolecularCloning]
  2. Error fetching PMID 1943786: [Hanahan91]
  3. Error fetching PMID 2235524: [Nishimura90]
  4. US Patent 4,851,348 Media:pat4851348.pdf [Hanahan89]
  5. US Patent 4,981,797 Media:pat4981797.pdf [Jessee90]
  6. US Patent 6,247,369 Media:pat6274369.pdf [Donahue01]
  7. US Patent 6,706,525 Media:pat6706525.pdf [Greenr04]
  8. US Patent 6,709,854 Media:Pat6709854.pdf [Donahue04]
  9. US Patent 6,709,852 Media:pat6709852.pdf [Bloom04]
  10. US Patent 6,855,494 Media:pat6855494.pdf [Bloom05]
  11. US Patent 6,960,464 Media:pat6960464.pdf [Jessee05]
  12. Error fetching PMID 4559594: [Cohen-PNAS-1972]
    This appears to be one of the earliest papers on transformation of Escherichia coli. If you find an earlier paper, please include it here.

All Medline abstracts: PubMed HubMed
Personal tools