Banta:AKR

Protein and Metabolic Engineering

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Altering the Substrate and Cofactor Specificities of a Dehydrogenase

Engineering substrate and cofactor specificity of a thermostable alcohol dehydrogenase.

Related Publications

1. Campbell E, Wheeldon IR, and Banta S. Broadening the cofactor specificity of a thermostable alcohol dehydrogenase using rational protein design introduces novel kinetic transient behavior. Biotechnol Bioeng. 2010 Dec 1;107(5):763-74. DOI:10.1002/bit.22869 |
2. Sanli G, Banta S, Anderson S, and Blaber M. Structural alteration of cofactor specificity in Corynebacterium 2,5-diketo-D-gluconic acid reductase. Protein Sci. 2004 Feb;13(2):504-12. DOI:10.1110/ps.03450704 |
3. Banta S, Boston M, Jarnagin A, and Anderson S. Mathematical modeling of in vitro enzymatic production of 2-Keto-L-gulonic acid using NAD(H) or NADP(H) as cofactors. Metab Eng. 2002 Oct;4(4):273-84. DOI:10.1006/mben.2002.0231 |
4. Banta S and Anderson S. Verification of a novel NADH-binding motif: combinatorial mutagenesis of three amino acids in the cofactor-binding pocket of Corynebacterium 2,5-diketo-D-gluconic acid reductase. J Mol Evol. 2002 Dec;55(6):623-31. DOI:10.1007/s00239-002-2345-x |
5. Banta S, Swanson BA, Wu S, Jarnagin A, and Anderson S. Optimizing an artificial metabolic pathway: engineering the cofactor specificity of Corynebacterium 2,5-diketo-D-gluconic acid reductase for use in vitamin C biosynthesis. Biochemistry. 2002 May 21;41(20):6226-36. DOI:10.1021/bi015987b |
6. Banta S, Swanson BA, Wu S, Jarnagin A, and Anderson S. Alteration of the specificity of the cofactor-binding pocket of Corynebacterium 2,5-diketo-D-gluconic acid reductase A. Protein Eng. 2002 Feb;15(2):131-40. DOI:10.1093/protein/15.2.131 |