Barry Canton/Bacterial lysis: Difference between revisions
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Barry Canton (talk | contribs) (New page: I need to lyse some ''E. coli'' under denaturing conditions. I'm collecting my on how to best do that here. ==OWW protocols== #[[Sauer:Purification of His-tagged proteins/Denaturing prep...) |
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Revision as of 08:41, 26 March 2008
I need to lyse some E. coli under denaturing conditions. I'm collecting my on how to best do that here.
OWW protocols
- Sauer:Purification of His-tagged proteins/Denaturing prep - the lysis part of this should generally useful
- Knight:Purification of His-tagged proteins/Denaturing - slightly different lysis buffer
- RNA extraction - describes use of guanidine isothiocyanate
- Sauer:ClpX purification/Protocol 1
- Sauer:His6 ClpX purification
- Sauer:SspB purification - Tween
- Sauer:Lysing E. coli with Lysozymes - lysozyme methods for native purification
- Sauer:Expression/purification of 35S-Met proteins/Native purification - Imidazole
