Bateman:Tools: Difference between revisions
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== New RFP balancers == | |||
We are in the process of creating new balancer chromosomes carrying a novel RFP reporter construct driven by a fragment of an enhancer from the ''pointed'' gene. This fragment drives strong expression in the oenocytes and fat body and so can be used to easily identify larvae or adult flies carrying it under a dissecting microscope with fluorescence. So far we have created a CyO balancer carrying this reporter and are in the process of creating similar first and third chromosome balancers. | We are in the process of creating new balancer chromosomes carrying a novel RFP reporter construct driven by a fragment of an enhancer from the ''pointed'' gene. This fragment drives strong expression in the oenocytes and fat body and so can be used to easily identify larvae or adult flies carrying it under a dissecting microscope with fluorescence. So far we have created a CyO balancer carrying this reporter and are in the process of creating similar first and third chromosome balancers. | ||
[[Image:Oen.jpg|none|400px|thumb|(A) Third instar larvae carrying CyO-RFP showing strong expression in oenocytes. (B) Adult fly carrying CyO-RFP showing RFP expression in oenocytes]] | [[Image:Oen.jpg|none|400px|thumb|(A) Third instar larvae carrying CyO-RFP showing strong expression in oenocytes. (B) Adult fly carrying CyO-RFP showing RFP expression in oenocytes]] | ||
Revision as of 05:16, 17 March 2009
New RFP balancers
We are in the process of creating new balancer chromosomes carrying a novel RFP reporter construct driven by a fragment of an enhancer from the pointed gene. This fragment drives strong expression in the oenocytes and fat body and so can be used to easily identify larvae or adult flies carrying it under a dissecting microscope with fluorescence. So far we have created a CyO balancer carrying this reporter and are in the process of creating similar first and third chromosome balancers.
