PAGE IN CONSTRUCTION - PLEASE COME BACK SOON!! (AND FEEL FREE TO EDIT/ADD)
This page is meant to help beginners -typically undergraduate or graduate students and post-docs- willing to ascidian development using molecular tools to address questions about gene activities and/or tissue-specific developmental processes.
In this regard, I consider that you have access to gravid adult animals to perform the research.
fertilization, dechorionation and embryo culture
database search, sequence analysis and preparation of molecular tools
electroporation and microinjection of Ciona eggs
fixing, staining and mounting embryos