Benjamin Wang: Difference between revisions
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== Research Interests == | == Research Interests == | ||
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Inverse metabolic engineering involves generating a library of mutant strains and screening through these mutants to identify which genes are responsible for a particular phenotype. Since the size of a genetic library can be large, using a high throughput screening method is important. In the area of metabolic engineering, one typically screens for the concentration of the metabolite of interest secreted into the cell culture media. Commonly used tools to measure metabolite concentrations, such as HPLC, are slow. A measurement which could be used for high throughput screening is an enzyme electrode. In this technology, an enzyme is immobilized on the surface of an electrode. When the enzyme reacts with its specific substrate, electrons are transferred from the substrate to a cofactor associated with the enzyme which then generates a current through the electrode. I plan to develop a high throughput screening method using enzyme electrodes to screen a library of mutants for amino acid overproduction. | Inverse metabolic engineering involves generating a library of mutant strains and screening through these mutants to identify which genes are responsible for a particular phenotype. Since the size of a genetic library can be large, using a high throughput screening method is important. In the area of metabolic engineering, one typically screens for the concentration of the metabolite of interest secreted into the cell culture media. Commonly used tools to measure metabolite concentrations, such as HPLC, are slow. A measurement which could be used for high throughput screening is an enzyme electrode. In this technology, an enzyme is immobilized on the surface of an electrode. When the enzyme reacts with its specific substrate, electrons are transferred from the substrate to a cofactor associated with the enzyme which then generates a current through the electrode. I plan to develop a high throughput screening method using enzyme electrodes to screen a library of mutants for amino acid overproduction.<br> | ||
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Link to [[Stephanopoulos Lab]] | Link to [[Stephanopoulos Lab]] | ||
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Revision as of 13:49, 10 April 2006
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Benjamin Wang Laboratory for Metabolic Engineering and Bioinformatics Stephanopoulos Lab (617)253-6591
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Research Interests
Inverse metabolic engineering involves generating a library of mutant strains and screening through these mutants to identify which genes are responsible for a particular phenotype. Since the size of a genetic library can be large, using a high throughput screening method is important. In the area of metabolic engineering, one typically screens for the concentration of the metabolite of interest secreted into the cell culture media. Commonly used tools to measure metabolite concentrations, such as HPLC, are slow. A measurement which could be used for high throughput screening is an enzyme electrode. In this technology, an enzyme is immobilized on the surface of an electrode. When the enzyme reacts with its specific substrate, electrons are transferred from the substrate to a cofactor associated with the enzyme which then generates a current through the electrode. I plan to develop a high throughput screening method using enzyme electrodes to screen a library of mutants for amino acid overproduction.
Link to Stephanopoulos Lab
Education
1997 B.S. in Chemical Engineering, Carnegie Mellon University
