Benjamin Wang: Difference between revisions
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<font size="4">Benjamin Wang</font size> | <font size="4">Benjamin L. Wang</font size> | ||
<font size="2">Laboratory for Metabolic Engineering and Bioinformatics | <font size="2">Laboratory for Metabolic Engineering and Bioinformatics<br> | ||
Department of Chemical Engineering<br> | Department of Chemical Engineering<br> | ||
77 Massachusetts Ave.<br> | 77 Massachusetts Ave.<br> | ||
56-422<br> | 56-422<br> | ||
Cambridge, MA 02139<br> | Cambridge, MA 02139<br> | ||
Phone: (617)253-6591<br> | |||
(617)253- | Fax: (617)253-7181<br> | ||
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== Research Interests == | == Research Interests == | ||
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Inverse metabolic engineering involves generating a library of mutant strains and screening through these mutants to identify which genes are responsible for a particular phenotype. Since the size of a genetic library can be large, using a high throughput screening method is important. In the area of metabolic engineering, one typically screens for the concentration of the metabolite of interest secreted into the cell culture media. Commonly used tools to measure metabolite concentrations, such as HPLC, are slow. A measurement which could be used for high throughput screening is an enzyme electrode. In this technology, an enzyme is immobilized on the surface of an electrode. When the enzyme reacts with its specific substrate, electrons are transferred from the substrate to a cofactor associated with the enzyme which then generates a current through the electrode. I plan to develop a high throughput screening method using enzyme electrodes to screen a library of mutants for amino acid overproduction.<br> | |||
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== | == Work Experience== | ||
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Jan. 2003 - June 2004 Hitachi Global Storage Technologies<br> | |||
June 1997 - Dec. 2002 IBM<br> | |||
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== Publications == | == Publications == | ||
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<biblio> | <biblio> | ||
</biblio> | </biblio> | ||
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<big>'''''Return to [[Stephanopoulos Lab]] OWW Home Page'''''</big> | |||
Latest revision as of 14:20, 10 April 2006
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Benjamin L. Wang Laboratory for Metabolic Engineering and Bioinformatics
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Research Interests
Inverse metabolic engineering involves generating a library of mutant strains and screening through these mutants to identify which genes are responsible for a particular phenotype. Since the size of a genetic library can be large, using a high throughput screening method is important. In the area of metabolic engineering, one typically screens for the concentration of the metabolite of interest secreted into the cell culture media. Commonly used tools to measure metabolite concentrations, such as HPLC, are slow. A measurement which could be used for high throughput screening is an enzyme electrode. In this technology, an enzyme is immobilized on the surface of an electrode. When the enzyme reacts with its specific substrate, electrons are transferred from the substrate to a cofactor associated with the enzyme which then generates a current through the electrode. I plan to develop a high throughput screening method using enzyme electrodes to screen a library of mutants for amino acid overproduction.
Education
1997 B.S. in Chemical Engineering, Carnegie Mellon University
Work Experience
Jan. 2003 - June 2004 Hitachi Global Storage Technologies
June 1997 - Dec. 2002 IBM
Publications
Return to Stephanopoulos Lab OWW Home Page
